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題 名 | 臺灣獼猴冷凍精液保存的探討=The Study of Semen Cryopreservation of Formosan Macaques (Macaca Cyclopis) |
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作 者 | 季昭華; 朱俊享; 林安仲; | 書刊名 | 動物園學報 |
卷 期 | 10 1998.06[民87.06] |
頁 次 | 頁43-50 |
分類號 | 383.16 |
關鍵詞 | 精液冷凍保存; 臺灣獼猴; Semen cryopreservation; Formosan macaque; Macaca cyclopis; |
語 文 | 中文(Chinese) |
中文摘要 | 精子冷凍保存技術的應用,有利瀕臨絕種動物及動物園內野生動物的物種保存, 如優良品種的保存及改良、計劃性繁殖的進行、減少動物因繁殖需要搬動而導致的死亡或 受傷及避免疾病的導入。人類及非人靈長類動物的精子冷凍保存雖已進行多年, 但非人靈 長類精子冷凍後的人工授精成功率卻不高。而目前且無臺灣獼猴冷凍精液的研究,故本研 究為進行臺灣獼猴精液冷凍保存的探討,選用體重9—15kg之雄性獼猴5隻,以經肛門直 腸電激方式採集精液,然後將精子以5種不同的冷凍保護劑及3種冷凍方法進行實驗,並 比較解凍後及1 hr、2 hr、4 hr後精子生存率、前進運動率及活力。結果含7.5﹪甘油TYB 冷凍保護劑以Straw冷凍方法,精子之生存率、前進運動率及活力最佳。於解凍後有68﹪ 精子生存率、64﹪前進運動率及活力+++,但獼猴精子於解凍1小時後,其精子前進運 動率及活力均明顯下降。如何提昇並延長精子的受精能力是冷凍精子進行人工授精能否成 功的重要關鍵,故進一步評估及提昇臺灣獼猴精子於解凍後之受精能力是未來研究的重要 課題。 |
英文摘要 | The use of semen cryopreservation is beneficial for the conservation of endangered species and zoo animals, the improvement and diversification of their breeds, the programming of reproduction plans, the reduction of animal transportation for the sole purpose of breeding, and the minimization of the spreading infectious diseases. The semen cryopreservation of human and nonhuman primates has been studied for several years, but the sucessful rate for nonhuman primates is very low. In order to better understand semen cryopreservation of the Formosan macaque, five male Formosan macaques weighting from 9-15 kg were used. The electroejaculation was used to collect semen from the macaques. The semen with 5 different protectant fluids and 3 different cryopreservation methods was carried out. The sperm viability and motility and activity were examined, one, two, and four hours intervals after thawing. It was found the semen was remained most optimal conditions on 7.5% glycerol TYB protectant by straw cryopreservation and showing 65% progressive motility and + + + activity. But the progressive motility and activity became noticeably reduced after the one hour interval. How to promote and prolong the quality of the frozen semen is the key to the success of artificial insemination. The improvement of semen cryopreservation and artificial insemination of the Formosan macaque is an important goal of future research. |
本系統中英文摘要資訊取自各篇刊載內容。