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頁籤選單縮合
題名 | 生乳及少數其他動物來源金黃色葡萄球菌腸毒素類型之檢測=The Enterotoxigenic Types for 62 Staphylococcus Aureus Isolates from Raw Milk and Some Other Animal Specimens |
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作者姓名(中文) | 吳建志; 鄭純彬; 潘銘正; | 書刊名 | 藥物食品分析 |
卷期 | 5:2 1997.06[民86.06] |
頁次 | 頁145-150 |
分類號 | 412.37 |
關鍵詞 | 金黃色葡萄球菌腸毒素; 酵素連接免疫吸附法; 聚合酶鏈反應; Staphylococcal enterotoxins; Enzyme-linked immunosorbent assay; Polymerase chain reaction; |
語文 | 中文(Chinese) |
中文摘要 | 將76株分離自動物及其產製品之金黃色葡萄球菌(生乳62株、鯨魚3株、海豚4株、 乳房炎牛乳汁 2 株、豬 2 株,雞、鼠、犬各 1 株 ),以商品化之 ELISA 套組檢測其腸毒 素產生能力, 結果有 26 株產生 A 型腸毒素,產生 B、C 型腸毒素者分別有 2 株及 1 株 ,而未檢出產生 D、E 型腸毒素之菌株。另以自行設計之引子對 (primers),以毛細管進行 聚合�t鏈反應 (polymerase chain reaction, PCR) 檢測帶有產生 A 型腸毒素基因之菌株 ,結果亦有 26 株呈陽性反應, 與 ELISA 之檢測結果相同,但 PCR 檢測 A 型腸毒素基因 之靈敏度為 10 �崟FU/ml。 |
英文摘要 | A commercial ELISA kit (RIDASCREEN�� SET A, B, C, D, E. R-Biopharm GmbH, Darmstadt, Germany) was used to examine the enterotoxigenecity of 76 Staphylococcus aureus strains. Of these strains, 62 were isolated from raw milk, 3 from whale, 4 from dolphin, 2 from cow milk with mastitis, 2 from pig, and 1 each from chicken, mouse, and dog. Type A, B, and C enterotoxins were detected in 26, 2, and 1 strains, respectively. Neither type D nor type E enterotoxin-producing strain was observed. In addition, DNA amplification by capillary polymerase chain reaction (PCR) with specific primers was used to detect the presence of enterotoxin A gene. The results showed that 26 strains harbored this gene. Study on the detection sensitivity of PCR indicated that the lowest cell number required for positive reaction was 10 �� CFU/ml. |
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