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題名 | Excessive Expression of the Tumor Necrosis Factor-α Gene in the Kidneys of Patients with Membranous Glomerulonephritis=膜性腎絲球腎炎患者的腎組織表現出高頻率的α腫瘤壞死因子基因 |
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作者 | 吳采虹; 蔡長祐; 楊五常; | 書刊名 | 中華醫學雜誌 |
卷期 | 61:9 1998.09[民87.09] |
頁次 | 頁524-530 |
分類號 | 415.812 |
關鍵詞 | 細胞激素; 介白質; 膜性腎絲球腎炎; 極微小變化腎病; 反轉錄酶-DNA聚合酶鏈反應; α腫瘤壞死因子; Cytokine; Interleukin; Membranous glomerulonephritis; Minimal change disease; Reverse transcriptase-polymerase chain reaction; Tumor necrosis factor-α; |
語文 | 英文(English) |
中文摘要 | 背景 膜性腎絲球腎炎以及極微小變化腎病的致病機轉還不太清楚,而細胞激素 在其中的角色也不甚明確。本實驗乃採用反轉錄��-DNA聚合�蓇鴗狨釭漣瑋N,分析膜性腎 絲球腎炎、極微小變化腎病以及各式腎絲球疾病患者的腎組織中,細胞激素基因的表現情形, 試圖了解細胞激素在這類疾病中所扮演的角色。 方法 選擇進行中或活動期膜性腎絲球腎炎、極微小變化腎病、局部扇形腎小球硬化症、急 性腎小管間質腎炎、糖尿病性腎病、免疫球蛋白A腎病、高血壓性腎埂化、免疫球蛋白M腎 病、類澱粉症以及末期腎病合併腎絲球硬化等患者的腎組織,進行反轉錄��-DNA累合醄鏈 反應,以分析其中細胞激素基因的表現情形;這些激素包括α腫瘤壞死因子、第一、二、四、 八及十介白質。 結果 膜性腎絲球腎炎患者的腎組織中,α腫瘤壞死因子基因的表現頻率較其他患者為高(p =0.0295),其基因表現在極微小變化腎病的患者中也較高,但未達統計學上有意義的程度 (p=0.0794)。另外,有2位極微小變化腎病患者的周邊血液淋巴球,其第四介白質基因不 論該細胞是否受到商陸刺激素(pokeweed mitogen)的刺激,皆會大幅度表現出來。 結論 總括而言,α腫瘤壞死因于基因在膜性腎絲球腎炎和極微小變化腎病的患者腎組織中 有高頻率的表現。而第四介白質基因則在極微小變化腎病患者周邊血液的淋巴球中有異常的 表現。此種結果顯示:α腫瘤壞死因子及第四介白質可能在膜性腎絲球腎炎和極微小變化腎 病病灶形成的過程中,直接或間接地扮演了某種角色。 |
英文摘要 | Background. The implication of cytokines in the pathogenesis of membranous glomeru-lonephritis (MGN) and minimal change disease (MCD) is not well known. To delineate the pathogenic roles of cytokines in MCN and MCD, renal tissue from patients with a variety of glomerulonephritides was studied for cytokine mRNA expression. Methods. The renal tissue from patients with MGN, MCD, focal segmental glomerulo- sclerosis, acute tubulointerstitial nephritis, diabetic nephropathy, immunoglobulin (lg) A nephropathy, hypertensive nephrosclerosis, lgM nephropathy, amyloidosis and glomerulosclerosis were studied for the expression of cytokines (tumor necrosis factor, [TNF]-α, interleukin [IL]-lβ, IL-2, IL-4, IL-8 and IL-1O) by reverse transcriptase polymerase chain reaction. Results. The frequency for renal expression of TNF-a was significantly higher in patients with MGN than in patients with other diseases (p = 0.0295, as analyzed by Fisher's exact test). Its expression in MCD was also more frequent than in the controls, although not significant (p=0.0794). IL-4 transcript was demonstrated in cultured lymphocytes stimulated with or without pokeweed mitogen in two patients with MCD. Conclusions. TNF-α, which is mainly produced by macrophages and neutrophils, is excessively expressed in the kidneys of patients with active MCN and MCD. IL-4, on the other hand, is expressed in the lymphocytes of patients with MCD. These results suggest that TNF-α and IL-4 may be involved directly or indirectly in the pathogenesis of these two diseases. |
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