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題名 | Genetic Variation in the Intergenic Spacer of Ribosomal DNA of Imperata Cylindrica (L.) Beauv. var. Major (Cogongrass) Populations in Taiwan=臺灣地區白茅族群核糖體核酸基因間隔區之遺傳變異 |
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作者 | 周昌弘; 蔡奇助; Chou, Chang-hung; Tsai, Chi-chu; |
期刊 | Botanical Bulletin of Academia Sinica |
出版日期 | 19991000 |
卷期 | 40:4 1999.10[民88.10] |
頁次 | 頁319-327 |
分類號 | 436.1 |
語文 | eng |
關鍵詞 | 基因間隔區; 核糖體核酸; 白茅; Cogongrass; IGS; Imperata cylindrica; PCR-amplified RFLP; rDNA; |
中文摘要 | 利用 PCR-amplified RFLP 分析核糖體 (rDNA) 核酸基因間隔區 (intergenic spacer, LIGS), 以探討台灣白茅族群之遺傳變異。在 17S rRNA 基因區的 3' 端與 25S rRNA 基因區的5' 端各設計一條引子, 利用 PCR 技術將來自 15 族群的 45 個白茅樣本之 IGS 進行複製 ,共可發現 5 種不同長度的 IGS 區域, 分別為 2730bp , 2830bp, 2930bp, 3030bp 和 313Obp, 其中以 2930bp 為白茅 IGS 的最主要型式。另外在單一白茅個體中亦可發現 1 至 2 種不同長度的 IGS 。上述 PCR 產物再經 13 種限制酵素切割, 共可 記錄 283 條帶 , 其中有 248 條帶具多型性。經群叢分析後, 可將台灣地區白茅分成二群, 一群為竹圍族群; 另一群為其它地區白茅族群。由上述研究可知,IGS 經 PCR-amplified RFLP的分析,不僅可獲取不少遺傳標誌,在微演化的研究上亦有幫助。 |
英文摘要 | Genetic variation in the intergenic spacer (IGS) of ribosomal DNA (rDNA) repetitive sequences in interand intra-specific populations of Cogongrass [Imperata cylindrica (L.) Beauv. var. major (Nees) Hubb] in Taiwan, was studied by employing PCR-amplified RFLP analysis. Each IGS region of 45 individuals from 15 populations was amplified using a pair of complementary primers to conserved regions in the 5'end of 17S and 3'end of 25S rRNA genes. Five IGS length variants were found at 2,730 bp, 2,830 bp, 2,930 bp, 3,030 by and 3,130 bp. Of them, 2930 by was the main type of IGS length of Cogongrass in Taiwan. Within an individual Cogongrass, one to two IGS length types were distinguished. Those samples amplified by PCR were digested with 13 restriction enzymes, and 283 bands were revealed. Of them, 248 bands were polymorphic. Clustering analysis was conducted based on data obtained from bands distributed in all samples mentioned, and two major clusters were found. The Chuwei population was distinctly different from the remaining populations. Moreover, the findings of the PCR-amplified RFLP analysis also indicated that the IGS region of rDNA provides a good genetic marker and a potential tool for the study of micro evolutionary process. |
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