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| 題 名 | 利用固相萃取及氣相層析法分析番木瓜中苄基異硫氰酯含量之研究=Determination of Benzyl Isothiocyanate in Papaya Fruit by Solid Phase Extraction and Gas Chromatography |
|---|---|
| 作 者 | 許輔; 徐源泰; | 書刊名 | 藥物食品分析 |
| 卷 期 | 4:4 1996.12[民85.12] |
| 頁 次 | 頁327-334 |
| 分類號 | 412.37 |
| 關鍵詞 | 苄基異硫氰酯; 固相萃取; 番木瓜; Benzyl isothiocyanate; Solid phase extraction; Papaya; |
| 語 文 | 中文(Chinese) |
| 英文摘要 | In this study, a method for benzyl isothiocyanate (BITC) determination in papaya samples was established by solid phase extraction and GC analysis. The adsorption as well as the elution of BITC in a commercial C18 cartridge are discussed. The maximum theoretical preconcentration factor calculated from the breakthrough curve and elution curve was 1.5 (based on simultaneous samples containing 1,000 ppm BITC). The lowest detectable level was 100ppb, and the linearity was acceptable. Four cultivars of papaya fruits were analyzed and the BITC contents ranged from 141.7-342.7 ppm in seed, 23.3-45.1 ppm in pericarp, 21.2-43.1 ppm in pulp, and 15.2 to 19.8 ppm in papaya milk. The average recovery was 95.1%. Results showed the advantages of solid phase extraction of BITC: easy and rapid operation, higher recovery, and the use of less solvent, comparing to conventional solvent extraction method. |
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