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題名 | 腸毒素型大腸桿菌之質體分析及熱穩定性腸毒素STI基因之聚合酶鏈反應檢測=Plasmid Profile Analysis for Enterotoxigenic Escherichia Coli and Detection of Heat Stable Enterotoxin I (STI) Gene by Polymerase Chain Reaction |
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作者姓名(中文) | 曾浩洋; 季萬榮; | 書刊名 | 藥物食品分析 |
卷期 | 4:3 1996.09[民85.09] |
頁次 | 頁215-222 |
分類號 | 412.37 |
關鍵詞 | 腸毒素型大腸桿菌; 質體DNA; 熱穩定性腸毒素; 聚合酶鏈反應; Enterotoxigenic E. coli; Plasmid DNA; Heat stable toxin I; PCR; |
語文 | 中文(Chinese) |
英文摘要 | Enterotoxigenic Escherichia coli (ETEC) is one of the food pathogens which may cause diarrhea in humans. The enterotoxins produced by ETEC are heat labile toxins (LT) and heat stable toxins (ST). These pathogenic factors are usually encoded on the plasmid DNA of E. coli cells. In this study, E. coli plasmid DNA was isolated and subjected to 0.7% agarose gel electrophoretic analysis and the STI gene was detected by polymerase chain reaction (PCR) using new primers. It was found that most of the plasmids from ETEC cells showed molecular weights in the range of 2-22 kb and the PCR primers used specifically amplified a 203 bp fragment. |
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