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題名 | 乳牛αs1--酪蛋白基因組DNA 1.0PP片段重複序列之研究=Studies on the Repetitive Sequence of 1.0PP Fragment in Dairy Catte αs1-Casein Genomic DNA |
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作者姓名(中文) | 陳慶任; 黃木秋; 李春進; | 書刊名 | 農林學報 |
卷期 | 45:2 1996.06[民85.06] |
頁次 | 頁15-22 |
分類號 | 437.21 |
關鍵詞 | αs1-酪蛋白基因組DNA; 重複序列; DNA指紋; αs1-casein genomic DNA; Repetitive sequence; DNA fingerprint; |
語文 | 中文(Chinese) |
中文摘要 | 真核生物基因組DNA中含有很多的重複序列。以經限制�t切割之基因組DMA,在瓊 脂糖膠體中電泳後,與同位素標幟的重複序列探針雜交,可以形成 DNA 指紋。 本研究室選 殖之 pBo1.0PP 重組質體, 係將長約 1.0 kb 之 Aryshire 乳牛之α�輊窗邿T蛋白基因組 DNA1.0PP 片段(此片段定名為 1.0PP 片段)構築在質體 pUC18 中。以雙去氧 DNA 序列分 析法分析 1.0PP 片段之核��酸序列,結果發現其內散佈著 4 段重複序列,其氮基之組成屬 GC rich 序列性質。在這些重複序列中,尚有 2 個 TTCTCC 及 3 個 AAGTGA 之小段重複序 列存在。同一個體之荷蘭種乳牛基因組 DNA 以不同限制�t切割,在 0.8 %瓊脂糖膠體電泳 後,與經�e P 標幟之 1.0PP 片段 DNA 探針雜交後,出現環帶之多態性,顯示 1.0PP 片段 具供建立荷蘭種乳牛 DNA 指紋圖譜探針用之價值。 |
英文摘要 | Repetitive sequence often represents a major component of the eukaryotic genomic DNA. Radio-labeled repeat sequence has been used as a probe to hybridze with restiction enzyme digested genomic DNA in agarose gel for DNA fingerprinting. The pBo1.0PP recombinant plasmid DNA containing a Aryshire α�� �� -casein genomic DNA about 1.0Kb, designated 1.0PP fragment, which was cloned and constructed into the PstI site of pUC18 was prepared for nucleotide sequencing by dideoxy DNA sequencing method. The result showed that there were 4 repetitive sequences in the 1.0PP fragment. It was found the base composition of these repetitive sequences was GC rich. Two TTCTCC and three AAGTGA short repeat sequences existed in the 1.0PP fragment. The same individual genomic DNA of Holstein cattle digested with different kinds of restrictionenzymes was run in 0.8% agarose gel and hybridized with 32p-labeled 1.0PP fragment DNA probe. The autoradiogram revealed polymorphism of bands, it showed that the 1.0PP fragment can be used as a hybridization probe for DNA fingerprinting. |
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