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頁籤選單縮合
題名 | 以重複序列探針建立荷蘭種乳牛DNA指紋=The Probe of Repeatitive Sequence for DNA Fingerprinting in Holstein Cattle |
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作者 | 蕭振文; 黃木秋; | 書刊名 | 中華農學會報 |
卷期 | 177 1997.03[民86.03] |
頁次 | 頁1-10 |
分類號 | 437.3 |
關鍵詞 | 荷蘭種乳牛; 重複序列探針; DNA指紋; Holstein cattle; Repeatitive sequence; DNA fingerprints; |
語文 | 中文(Chinese) |
中文摘要 | 本試驗之目的是利用重複序列寡核苷酸(TG)₆探針,建立荷蘭種乳牛DNA指紋圖譜。自荷蘭種乳牛之頸靜脈採血,離心收集白血球供基因組DNA萃取之用。基因組DNA以限制酶HinfI或HaeIII切割後,在瓊脂糖膠體中進行電泳,將膠體乾燥後,與重複序列探針進行膠體原位雜交,然後以X-光片進行自動放射顯影。試驗結果顯示,荷蘭種乳牛之基因組DNA不論是以限制酶HinfI或HaeIII切割均可形成DNA指紋。基因組DNA以限制酶HaeIII切割者,其雜交後呈現之DNA指紋橫帶數目較多且解析度較HinfI切割者為佳。但以限制酶HinfI切割所形成之DNA指紋較以限制酶HaeIII切割者具多態性。公牛之DNA指紋具有一公牛所獨有而為母牛所無之明顯特異橫帶存在。其是否具有供開發為荷蘭種乳牛性別鑑定用DNA探針之價值,值得進一步深入之探討。 |
英文摘要 | The purpose of this stud was to investigate the DNA fingerprints of Holstein cattle using the probe of repeatitive oligonucleotide sequence (TG)₅. Genomic DNA of Holstein cattle was extracted from blood and digested with restriction endonucleases HinfI or HaeIII. The digested DNA was run in agarose gel and the gel was dried and hybridized with [8e65]P-labeled probe. Autoradiograms were made for DNA fingerprinting. The results showed that DNA fingerprints could be obtained from the treatments of genomic DNA either digested with HinfI or HaeIII. Much more bands of DNA fingerprints were observed in the genomic DNA digested with HaeIII than which digested with HinfI, but more polymorphism was found in the treatment of HinfI. The DNA fingerprints of Holstein cattle showed that only the male cattle presented a sex-specific band when their genomic DNA was digested with HaeIII and hybridized with repeatitive sequence probe. Further investigation to evaluate whether the male-specific DNA fragment being a potential probe for future application in sex determination of cow embryos is required. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。