頁籤選單縮合
題名 | Male-Specific Band in Random Amplified Microsatellite Polymorphism Fingerprints of Holstein Cattle=荷蘭種乳牛逢機增殖微衛星多態性指紋中公牛之特異環帶 |
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作者 | 洪炎明; 黃木秋; Horng, Yan-ming; Huang, Mu-chiou; |
期刊 | Proceedings of the National Science Council : Part B, Life Science |
出版日期 | 20000100 |
卷期 | 24:1 2000.01[民89.01] |
頁次 | 頁41-46 |
分類號 | 437.3 |
語文 | eng |
關鍵詞 | 荷蘭種乳牛; 逢機增殖微衛星多態性; 公牛; 特異環帶; Male-specific band; RAMPO; Holstein cattle; |
英文摘要 | A total of 65 blood samples collected from Holstein cattle were employed for DNA extraction. Genomic DNA were amplified by means of random amplified polymorphic DNA (RAPD). One hundred and one random primers (Operon kits OPAA, OPAO, OPAV, OPC, OPE and OPA-06) were used for polymerase chain reactions (PCR).The PCR products were size fractionated by means of electrophoresis in agarose gel, transferred to a nylon membrane, and then hybridized with a a-32P- labeled microsatellite probe for random amplified microsatellite polymorphism (RAMPO) analysis. All the primers produced many amplification patterns in RAMPO fingerprints, but only OPE-01 (5 CCCAAGGTCC-3 yielded a male-specific strong signal. Among the 65 cases, the specific band could be observed in the RAMPO fingerprints of the 24 male samples but not in the 41 female samples. Therefore, this specific band is peculiar to male cattle. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。