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題 名 | Polymorphisms of Twelve Short Tandem Repeat Loci in a Taiwanese Population and Their Application in Parentage Testing=中國人之十二個短重複序列位點的基因分析,及其於親子鑑定之應用 |
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作 者 | 曾成槐; 劉昭儀; 陳瀅如; 胡惠宇; 林炯熙; 雍建輝; 朱廣邦; 王聲遠; 李俊億; | 書刊名 | 臺灣醫學會雜誌 |
卷 期 | 97:11 1998.11[民87.11] |
頁 次 | 頁738-744 |
分類號 | 362.6 |
關鍵詞 | 中國人; 短重複序列位點; 基因分析; 親子鑑定; Short tandem repeats; Polymerase chain reaction; Cumulative power of exclusion; Probability of paternity; |
語 文 | 英文(English) |
英文摘要 | With the advancement of techniques in molecular biology, rapid, sensitive, and reliable methods of DNA typing for parentage testing have become available. In this study, we evaluated the usefulness of multiplex polymerase chain reaction (PCR) with 12 unlinked short tandem repeat (STR) loci for paternity testing in Taiwan. The genetic informativeness of this test was then compared with that of conventional human leukocyte antigen(HLA) analysis in 167 parentage studies. The 12 STR loci alone provided a cumulative power of exclusion of up to 0.9998. Paternity was excluded in 59 (35.3%) cases, including 40 of 112 paternity trios and 19 of 55 paternity duos. In the 40 trios in which paternity was excluded, a mean of 6 (range, 3-9) incompatible STR markers were in the 19 duos in which paternity was excluded, a mean of 4 (range, 1-8) incompatible STR marker were noted. In the 72 trios in which the alleged paternity could not be excluded, the mean probabilities of paternity (PP) were 90.6863% with HLA testing alone, 99.9847% with STR analysis alone, and 99.9972% with combined HLA and STR analysis. In the 36 duos in which the alleged paternity could not be excluded, the mean PPs were 81.4768% with HLA testing alone, 99.6124% with STR analysis alone, and 99.9145% with combined HLA and STR analysis. These results suggest that STR analysis is very powerful when used alone for paternity trio testing and when combined with conventional serologic HLA typing for duo parentage testing in the Taiwan population. |
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