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- Cloning and Restriction Mapping of Goat β-Casein Gene
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頁籤選單縮合
題名 | Cloning and Restriction Mapping of Goat β-Casein Gene=山羊β-酪蛋白基因之選殖及限制酶圖譜分析 |
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作者姓名(中文) | 黃木秋; 林志生; | 書刊名 | 農林學報 |
卷期 | 45:1 1996.03[民85.03] |
頁次 | 頁83-93 |
分類號 | 437.27 |
關鍵詞 | 限制酶圖譜分析; 基因組DNA; 酪蛋白基因; Restriction mapping; Genomic DNA; Casein gene; |
語文 | 英文(English) |
中文摘要 | 酪蛋白基因之表現具有高度的階段性及組織專一性。它適合應用在哺乳動物基因 轉殖等之研究上。本試驗目的在選殖出山羊β-酪蛋白基因,期能供今後從事基因表現及轉 殖等研究之用。利用原位溶菌斑雜交方法自山羊基因組集合庫中篩選出含有山羊β-酪蛋白 DNA 片段之陽性殖系, 再以 SalI 自陽性殖系中切出山羊β-酪蛋白 DNA 片段,將分離出 之片段接入 pUC 質體中供限制�t圖譜及序列分析用。 長度約 20kb 之山羊β-酪蛋白基因 組 DNA 的限制�t圖譜已經被定出來, 此一圖譜與已發表之牛的β-酪蛋白基因比較結果發 現彼此間有極高之相似性。其起動子之核��酸序列與牛之β-酪蛋白基因者間有 94 %之相 似性;第七個外顯子與牛者之間亦有高達 90 %以上之相似性。這些結果顯示選殖到之山羊 β-酪蛋白基因組 DNA 可能涵蓋了整個山羊β-酪蛋白基因序列。 |
英文摘要 | The casein gene has a highly stage- and tissue-specific expression. It is suitable for application in investigations of transgenic animals. Experiments were conducted to screen the goat β -casein gene for the use of gene expression and transfer experiments in the future. Positive clones of recombinant phages containing goat β -casein DNA fragments were isolated from a goat genomic library by plaque hybridizationin in situ. These clones were digested with SalI, the goat β -casein DNA fragments were constructed into pUC plasmids for restriction enzyme mapping and sequencing. A restriction enzyme map of goat β -casein genomic DNA that involved about 20kb was made. On comparing the restriction enzyme cutting sites in goat β -casein genonic DNA cloned in these experiments and bovine β -casein gene, we found that great similarities exist between them. The similarity of the promoter sequences both of them are 94%. More than 90% homologues of the seventh exon sequences of β -casein gene between goat and bovine was found. The goat β -casein genomic DNA may cover the entire goat β -casein gene. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。