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頁籤選單縮合
題 名 | 山羊β-酪蛋白基因組DNA之定序=Sequencing of the Goat β-Casein Genomic DNA |
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作 者 | 趙俊炫; 黃木秋; | 書刊名 | 中華農學會報 |
卷 期 | 179 1997.09[民86.09] |
頁 次 | 頁1-14 |
分類號 | 437.58 |
關鍵詞 | 基因組DNA; β-酪蛋白基因; 定序; Genomic DNA; β-casein gene; Sequencing; |
語 文 | 中文(Chinese) |
中文摘要 | β-酪蛋白為哺乳動物乳蛋白成分之一,其基因之表現具高度階段性與組織專一性 。為了提供基因轉殖動物研究及探討基因表現調控之需,本研究室已自基因組集合庫中選殖 出山羊β-酪蛋白基因。 本試驗之目的是將選殖到之山羊β-酪蛋白基因組 DNA 定序。定 序時, 係自 pGB5-1 及 pGB6-1 兩個重組質體中之山羊β-酪蛋白基因組 DNA 以適當之限 制�t切割並分殖入 pUC18 及 pGEM7Zf(+) 質體中,再直接或以 DNA 刪除作用後或使用合成 的寡核��酸引子,以雙去氧核��酸序列分析法定序。已將山羊β-酪蛋白基因序列 5' 端側 區 3417 bp、3' 端側區序列 120bp 及構造性基因序列 7091bp 定序,共定序 10628bp。起 動子及構造性基因序列之顯現子序列已全部定序清楚。將所得之序列與前人所發表之部分山 羊β-酪蛋白基因組 DNA 序列比對結果,發現有數處差異。 其中 5' 端側區序列有 18 處 ,介入子序列方面有 21 處,第七顯現子內有一處差異。這些差異或許為品種或個體差異所 造成。 |
英文摘要 | β-casein is a milk protein in mammalian milk. β-casein gene expression possess high stageand tissue-specific properties. For the studies of transgenic animal and gene expression, the goat β -casein gene had been cloned byour laboratory from a goat genomic library. The purpose of this study was focused on the sequencing of goat β -casein gene. The plasmids pGB5-1 and pGB6-1 containing goat β -casein genomic DNA were cut with restriction enzymes and subcloned into pUC18 or pGEM7Zf(+) for sequencing by dideoxy nucleotide sequencing method. The nuleotide sequences of 3417 bp of the 5'-flanking region containing the entire promoter sequence, 120 bp of the 3'-flanking sequence and 7091 bp of the β -casein stucture gene including all 9 exons had been determined. These results showed that there are 18 positions in the 5'-flanking region, 21 positions in intron sequence and 1 position in exon Ⅶ in conflict with the report of Roberts et al (1992). The different species or individuals maycontribe to these results. |
本系統中英文摘要資訊取自各篇刊載內容。