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題名 | 臺北市立動物園長臂猿種緣關係之基因分析=Phylogenetic Analysis of Gibbons at Taipei Zoo |
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作者 | 胡光宇; 林念修; 楊翕雯; Hu, Kuang-yu; Lin, Nien-hsiu; Yang, Ci-weu; |
期刊 | 動物園學報 |
出版日期 | 20000900 |
卷期 | 12 2000.09[民89.09] |
頁次 | 頁13-23 |
分類號 | 389.9 |
語文 | chi |
關鍵詞 | 長臂猿; 粒線體DNA; 聚合酵素連鎖反應; 定序; 種源關係; Gibbons; Mitochondrial DNA; Polymerase chain reaction; Sequencing; Phylogeny; |
中文摘要 | 為了確認臺北市立動物園內長臂猿的種別,本研究將長臂猿毛囊細胞內粒線體中細胞色素b(cytochrome b)的252鹼基以及第4組和第5組NADH脫氫酵素(NADH dehydrogenase cornp1ex)基因的896鹼基片段進行聚合酵素連鎖反應後直接定序。根據基因分類的結果顯示,可以鑑別動物園所飼養之長臂猿有H. leucogenys(中國白頰長臂猿)一頭,H. gabriellae(金頰長臂猿)二頭,H. muelleri(灰長臂猿)四頭,H. agilis(黑手長臂猿)四頭,H. lar(白手長臂猿)十七頭,H. syndactylus(大長臂猿)一頭,及一頭疑為雜交種之H. Klossii(小長臂猿)。園外所取得3隻長臂猿分別為H. muelleri一頭和H. leucogenys二頭。依據此研究之結果與外觀分類結果比對,除了名叫春花(G2)的長臂猿外,其餘基因分 類結果及外觀分類結果一致,並確認出外觀分類無法確定種別的幾隻長臂猿,包括G11、G13、G15、G16、G18及G25等。綜合基因與外觀分類所得的結果,顯示動物園的圈養記錄有9隻長臂猿的種別需要修正, 此一研究結果將有助於動物園長臂猿物種繁殖計畫之執行。 |
英文摘要 | To identify the species of gibbons of the Taipei Zoo, mitochondrial DNAs from their hair follicle samples were isolated and subjected to polymerase chain reaction (PCR) -sequencing. DNA sequences for both the 252-base-pair (bp) segment of the cytochrome b gene and the 896-bp fragment of ND4 and ND5 ( NADH Dehydrogenase complex) genes were successfully determined and used to construct phylogenetic trees. Preliminary genetic data indicate that 30 gibbons at the Taipei Zoo are 1 H. leucogenys, 2 H. gabriellae, 4 H. muelleri, 4 H. agilis, 17 H. lar, 1 H. syndactylus, and one suspected hybrid of H. klossii. In addition, 3 living specimens outside the Taipei Zoo are 1 H. muelleri and 2 H. leucogenys. Except for the gibbon G2, the molecular analysis is consistent with morphological analysis. Moreover, for gibbons G11, G13, G15, G16, G18, and G25, which were doubtfully identified by morphological analysis, molecular analysis provides a method of choice for classification. Those findings indicate that 9 of the gibbons housed at the Taipei Zoo were incorrectly identified. The result derived from this study will be essential to the success of the Taipei Zoo Gibbon Conservation Project. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。