頁籤選單縮合
| 題 名 | 毛豆花葯培養癒合組織之切片觀察及繼代培養 |
|---|---|
| 作 者 | 韓青梅; 陳庚鳳; 洪啟善; | 書刊名 | 高雄區農業改良場研究彙報 |
| 卷 期 | 6:2 1995.06[民84.06] |
| 頁 次 | 頁1-9 |
| 分類號 | 434.217 |
| 關鍵詞 | 毛豆; 花葯癒合組織; 繼代培養; 石蠟切片; Vegetable soybean; Anther callus; Subculture; Paraffin section; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 毛豆之花葯具有四個花粉囊,花粉囊相連處具有結締組織。毛豆花葯所產生之癒合組織經切片觀察結果得知,花葯培養形成之癒合組織除由花粉細胞增殖產生外,其他細胞如花葯壁或結締組織細胞均可增殖。因此癒合組織由何種細胞增殖而來目前從外表無法判定其來源,但由切片觀察獲知早期形成之癒合組織大多來自於體細胞,而花粉細胞形成之癒合組織出現較晚。如欲確定癒合組織之來源,尚待未來誘導成植株後以檢查根尖染色體判定之。癒合組織之繼代培養以MS基本培養基搭配5mg/L Kinetin 、0.05mg/L IAA、CW 150ml最適宜。 |
| 英文摘要 | Anatomical studies by paraffin section method for anther callus of vegetable soybean showed that there were four anther sac which connected with connective tissue, The cells in connective tissue, anther wall and pollen are able to divide to form callus. But we could hardly recognize the callus come from which kind of cells. Therefore, it is necessary to examine the chromosome number in root-tip cell when the callus regenerated to plantlet. The suitable medium for subculture of anther callus of vegtable soybean was MS basic medium with 5 mg/L Kinetin, 0.05 mg/L IAA and coconut water 150 ml/L. |
本系統中英文摘要資訊取自各篇刊載內容。