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題名 | 天麻藥材中指標成分Parishin, Parishin B及C之高效液相層析定量法之探討 |
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作者 | 顧祐瑞; 劉宜祝; 郝景平; 溫國慶; 林哲輝; 黃文鴻; | 書刊名 | 藥物食品分析 |
卷期 | 3:4 1995.12[民84.12] |
頁次 | 頁287-293 |
分類號 | 418.52 |
關鍵詞 | 天麻藥材; 高液相層析; 定量法; Gastrodiae rhizoma; Gastrodia elata blume; Parishin; Parishin B; Parishin C; HPLC; |
語文 | 中文(Chinese) |
英文摘要 | A rapid method by high performance liquid chromatography was developed for the simultaneous determination of three marker substances, parishin, parishins B and C, in Gastrodiae Rhizoma. This determination was carried out by using a gradient elution with methanol and 0.1% phosphoric acid on a Cosmosil 5C18-AR column. The linear calibration range of parishin, parishins B and C were 80~1200ug/ml (r=0.9998), 120~760ug/ml (r=0.9831) and 40~320 ug/ml(r=0.9995), respectively. The recoveries of the markers were parishin: 102.6±3.3%, parishin B: 102.8±2.7% and parishin C: 102.6± 2.9%. The relative standard deviations of the three marker substances for intraday and interday analyses were 0.73~2.11% and 0.67~3.56%, respectively. The contents of the markers mentioned above in 23 crude drugs of Castrodiae Rhizoma have been determined and the results were obtained as follows : parishin 0.37~1.50%, parishin B 0.12~0.65% and parishin C 0.08~0.27%. Therefore, HPLC is a useful technique to determine these selected markers. |
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