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頁籤選單縮合
題名 | 鹹可溶非色素食品添加物之高效液相層析定量法=Quantitative High Performance Liquid Chromatography of Several Alkaline-Soluble Non-Color Food Additives |
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作者 | 葉東柏; 郭建民; Yeh, Dong-bor; Kuo, Jen-kin; |
期刊 | 嘉南學報 |
出版日期 | 19961100 |
卷期 | 22 1996.11[民85.11] |
頁次 | 頁11-21 |
分類號 | 412.463、412.463 |
語文 | chi |
關鍵詞 | 食品添加物; 逆相C[feda]管柱; 高效液相層析定量法; Food additives; Reversed phase; C[feda]column; Quantitative HPLC; |
中文摘要 | 本報告提出一種可快速分析六種鹼可溶食品添加物,包括糖精、咖啡因、己二烯酸、 苯甲酸、乙期對羧苯甲醯酯及水楊酸的高效液相層析法。利用自行製備之短的(15×0.4cm)逆相 層析管柱(Lichrosorb RP-18),配合液相析儀,雙波長UV檢出儀及鬆筆記錄哈,上述六種添 加物可藉鈉-醋酸緩衝液(pH4.0),含4%異丙醇當做移種相,在15分鐘(流速為1.2mL/分)內 完全分離,而且吸收波峰高度與高效液相層析法濃度幾成正比,其靈每度可高至1~5ppm範 圍。 |
英文摘要 | A suitable procedure for the separation and quantitation of several alkaline-soluble food additives, including saccharin, caffeine, sorbic acid, benzoic acid, ethyl para-hydroxybenzoate and salicylic acid was worked out. In this study, the food additives tested were completely separated and distinguished within 15 minutes by a high pressure liquid chromatography through a small reversed phase column (LiChrosorb RP-18). An isocratic Na-acetate buffer (10%, pH4) containing 4% 2-propanol was used as eluent, and the minimum amount of sample required for assessment was in the nanogram range. Furthermore, by referring the elution profiles obtained from a dual-wavelength UV monitor and connected dual-pen recorder, the identification of additive (s) in food is more reliable than the one judged only by the retention time. |
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