查詢結果分析
來源資料
頁籤選單縮合
題 名 | Serodiagnosis of Tuberculosis by Enzyme-Linked Immunosorbent Assay for Anti-A60 and Anti-A38=用血清酵素免疫附著定量法來測定結核菌A60及A38抗體 |
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作 者 | 李立夫; 林孟志; 陳濘宏; 謝孟哲; 李政輝; 曹昌堯; | 書刊名 | 長庚醫學 |
卷 期 | 21:3 1998.09[民87.09] |
頁 次 | 頁258-264 |
分類號 | 415.2773 |
關鍵詞 | A38 IgG抗體; 酵素免疫附著定量法; A60 IgG; A60 IgM; A38 IgG antibodies; Enzyme-;inked immunosorbent assay; ELISA; |
語 文 | 英文(English) |
中文摘要 | 背景:血清酵素免疫附著定量法(ELISA)檢查是近來用來診斷肺結核的一個簡單 快速又便宜的方法。為了評估此方法的診斷價值,自1995年 4月至1996年 6月,我們在長庚 醫院使用血清酵素免疫附著定量法來做肺結核A60 IgG、IgM及A38 IgG抗體的測定。 方法:共有67個病人並被分成 3組:A組含24個肺結核痰液抹片陽性的病人。B組含18個肺結 核痰液抹片陰性的肺癌病人。C組含25個臨床表徵及胸部X光懷疑有肺結核感染,但痰液抹片 陰性或無痰可留供檢查者。 結果:在A60 IgG抗體方面,A、B及C組,全體之敏感度分別為91.7%、85.7%、89.5%,但準 確率皆偏低。在A60 IgM抗體方面,結果顯示有較低的敏感度(A、B及C組,全體分別為 37.5%、14.3%、28.9%),但有較高的特異性。A38 IgG抗體有較低的敏感度(40.0%、11.1%、 31.0%),但有較高的特異性(100%、71.4%、90.0%)。 結論:用A60 IgG抗體之酵素免疫附著定量法來診斷肺結核其敏感度高但特異性低,因而此 法可做為篩選臨床懷疑感染肺結核但無痰可留供檢查或痰液抹片陰性者之既快速又簡單之工 具。A60 IgM及A38 IgG抗體之酵素免疫附著定量法特異性高之性質,則於檢驗結果呈陽性時 可用以更確定肺結核之臨床診斷。總括言之,雖然酵素免疫附著定量法是一簡單快速又便宜 的肺結核篩選方法,用做診斷工具仍有其限制。 |
英文摘要 | Background: For early diagnosis of tuberculosis (TB), especially in the patients without adequate sputum specimens for examination, we found a simple, rapid and inexpensive method among many current available diagnostic tools, the enzyme-linked immunosorbent assay (ELISA). To investigate the diagnostic effectiveness of this method, we applied ELISA for detection of antigen 60 IgG and IgM as well as antigen 38 IgG antibodies at Chang Gung Memorial Hospital from April 1995 through June 1996. Materials and Methods: Sixty-seven patients were enrolled and divided into 3 groups, Group A (n=24), patients with positive sputum acid-fast stain; Group B(n=18), patients with lung cancer and negative sputum acit-fast stain; and Group C (n=25), patients with chest roentgenogram (CXR) which were suggestive of TB but with negative acid-fast stain results or no sputum for examination. Results: For the A60 IgG antibody, we found a sensitivity rate of 91.7% for Group A and Group B, and 85.7% for Group C as well as an overall sensitivity of 89.5% but with lower specificity. For the A60 IgM antibody, a lower sensitivity (37.5%, 14.3%, 28.9%, respectively) was found but with higher specificity. For the A38 IgG antibody, we found a lower sensitivity (40%, 11.1%, 31%, respectively) but with higher specificity (100%, 71.4%, 90%, respectively). Conclusion: With a high sensitivity but low specificity for diagnosis of TB, A60 IgG ELISA could be used as a rapid, simple screening test for patients with results suggestive of TB, especially in those who had no sputum or had negative sputum acid-fast stain results. Otherwise, A60 IgM or A38 IgG ELISA, with a high specificity, could be used as a reliable test in the diagnosis of pulmonary TB when the result is positive. In summary, although ELISA is a simple, rapid, inexpensive method, it is helpful but limited in the diagnosis of pulmonary TB. |
本系統中英文摘要資訊取自各篇刊載內容。