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| 題 名 | The Role of Immunohistochemical Estrogen Receptor Analysis in Cytologic Smear for Breast Cancer=乳癌女性激素受體免疫細胞化學分析研究 |
|---|---|
| 作 者 | 郭守仁; | 書刊名 | 中華民國外科醫學會雜誌 |
| 卷 期 | 26:6 民82.11-12 |
| 頁 次 | 頁2045-2049 |
| 分類號 | 416.226 |
| 關鍵詞 | 乳癌; 女性激素受體; 免疫細胞化學分析; Breast cancer; Immunohistochemistry; Monoclonal antibody; Estrogen receptor; |
| 語 文 | 英文(English) |
| 中文摘要 | 利用細針穿吸細胞檢查及蓋印式細胞檢查將過去一年(1990~1991)三十位乳癌病患包括晚期乳癌之細胞標本,利用女性激素荷爾蒙受體抗原之抗體,經過免疫細胞化學染色法,在顯微鏡下觀察測定乳癌細胞所含有之女性激素。荷爾蒙受體之量,我們是利用已製成之女性激素荷爾蒙受體抗原之抗體來從事此一研究,卅位乳癌中有廿三位抽吸或刮蓋上之細胞數足夠施行ERICA(女性激素荷爾蒙受體免疫細胞化學分析),而有十一位(46%),女性激素荷爾蒙受體為陽性反應,亦即在顯微鏡下我們可觀察到乳癌細胞核有紅棕色反應,廿三位可進行ERICA研究,乳癌病患中有六位是屬晚期乳癌(第三期或第四期),其中四位ER(-)(女性激素荷爾蒙受體陰性反應)。二位ER(+),呈陽性者對荷爾蒙治療反應極佳,而ER(-)之晚期乳癌對荷爾蒙治療則無療效反應,我們的結論是利用ERICA進行乳癌女性激素荷爾蒙受體測定,同樣可得滿意的結果,而由其結果之陰、陽性反應,可作為我們日後治療乳癌之指標及其預後之參考。 |
| 英文摘要 | Thirty patients with breast cancers, diagnosed in the period from July 1990 to May 1991 were studied to assess the reliability of immunocytochemical analysis of oestrogen receptor ( ER-ICA ) in specimens obtained by fine needle aspiration and touch imprint. Results were obtained by using the commercially available ERICA kit. An immuoperoxidase technique was utilized, with a monoclonal antibody to the estradiol receptor, to identify immunoreactive estradiol receptor in breast carcinomas. The relationship between the immunoreactive estradiol receptor and response to therapy was examined in patients with advanced breast cancer. Twenty-three of 30 cytological smears were evaluable. Under light microscopy estrogen receptors stained only in the nucleus of tumor cells, with a reddish-brown color. Negative and postive control slides provided in the ERICA kit were found to stain appropriately. Eleven of the 23 evaluable cases ( 46% ) were ER-positive. Six patients with advanced breast cancers were found to be assessable for response to endocrine therapy. Two, who showed an objective response to endocrine therapy, had positive-stained carcinomas. None of the four patients with negative-stained carcinomas responded to endocrine therapy. The couclusion: that ER-ICA is an alternative method to detect the Estrogen Receptor and can help to identify breast cancer patients who may response to endocrine manipulation. |
本系統中英文摘要資訊取自各篇刊載內容。