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頁籤選單縮合
題名 | 血清中牛奶與蛋成份IgE 及IgG 抗體之檢驗 |
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作者姓名(中文) | 張菊芬; 陳五常; 黃慶三; | 書刊名 | 慈濟醫學 |
卷期 | 4:2 1992.06[民81.06] |
頁次 | 頁85-93 |
分類號 | 412.37 |
關鍵詞 | 牛奶; 成份; 血清; 抗體; 蛋; 檢驗; |
語文 | 中文(Chinese) |
中文摘要 | 吾等選取166住過敏病人及226位健康人為對照組,各分成小於3歲,4-6 歲,7-10歲,11-16歲,大於16歲,五個年齡層。用ELISA方法測定其血清中對三 種牛奶過敏原β-lactoglobultn(BLG),α-lactalbumin(ALA),casein(CS)和蛋過敏原 ovalbumin(OA)的IgE與lgG抗體之濃度,找出血清最佳稀釋倍數以及各年齡層患者 對此四種過敏原的抗體含量情形。結果顯示,就血清稀釋倍數,三種牛奶過敏原 以10倍稀釋較佳,而蛋過敏原以100倍稀釋較好。各年齡層患者與對照組之間, 除了ALA外亦都有顯著差異。且各種抗體之含量在各年齡層之間亦有差異。 BLG,CS,大都是小於3歲時最高,然後隨年齡增加而降低。OA則是小於3歲時低, 然後慢慢升高至7-10歲後再往下降。本研究顯示,以適當的倍數稀釋過之血清, 採用ELISA法測定國人牛奶與蛋過敏原抗體,可瞭解這些抗體在不同年齡層的變 化情形。(慈濟醫學1992:4:85-93) |
英文摘要 | β-lactoglobulin (BLG), a-lactalbumin (ALA), casein (CS), and ovalbumin (OA) are food-antigenic proteins.We examined the serum levels of lgG andIgE against these proteins in 166 atopic patients and in 226 agematched normal controls. The antigens of BLG, CS, ALA and OA were coated on microtiter plates separately(5 ug/ml for each), then the diluted serum (1:10 or 1:100) were added. For lgG determination peroxidaseconjugated goat antihuman lgG then was labeled, while biotinated goat antihuman lgE and avidin-biotin-horseradish peroxidase were added for the measurement of lgE. The optical density at 490 nm was recorded for eachtest with an enzyme-immunoassay reader (Labsystems AUTO EIA). The results revealed that 1:10 serum dilution is better than 1:100 for BLG, ALA and CS, while 1:100 serum dilution is the better one for OA. Wedivided the patients into 5 age groups and found that both serum lgE and lgG against BLG, CS and OA weredifferent (P<0.05) from age-matched controls in every age group. The titer of serum lgE and lgG against BLG,CS, ALA declined with age, while OA increased after birth ui@til age 7 to 10 and declined thereafter in bothatopic patients and normal controls. We conclude that BLG, CS, OA, but not ALA, are helpful markers foratopic patients and 10 folds of dilution of serum is better in examining BLG and CS. (Tz'u-Chi Med J 1992;4:85-93) |
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