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題名 | 改進甘蔗嵌紋病病毒之純化 |
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作者 | 楊瓊儒; | 書刊名 | 臺中區農業改良場研究彙報 |
卷期 | 19 1988.06[民77.06] |
頁次 | 頁27-34 |
分類號 | 434.718 |
關鍵詞 | 甘蔗; 純化; 嵌紋病病毒; |
語文 | 中文(Chinese) |
中文摘要 | 甘蔗嵌紋病病毒分別以(A) Phosphate buffer, (B) Borate buffer及(C) Sodium citrate等三種萃取法純化後,發現以Sodium citrate萃取法之純化效果最 好,病毒液於蔗糖密度梯度離心呈現兩個A 260nm吸收高峰,靠近管底的高峰 A,病毒常纏繞結成團;靠近管中央的高峰B,病毒分散,無凝聚現象,雜質亦 少。其A 260/280之比值為1.20∼1.22。所得之純化病毒接種於玉米台南5號, 發病率達79%。 |
英文摘要 | The best sugarcane mosaic virus preparation was obtained from sodium citrate extraction method among the three methods used in this experiment including (A) phosphate buffer, (B) borate buffer and (C) sodium citrate. It showed two peaks (A and B) on the absorbance profile of sucrose density gradient centrifugation at the wavelength of 260 nm. Significantly aggregated virus particles were found in the fraction of absorption peak A near the bottom of the tube, while dispersed virus particles were condensed to the fraction of peak B near the center of the tube. The absorption ratio of 260/280 nm of the virus preparation ranged from 1.20-1.22. The purified virus showed an infectious rate of 79% on corn cv. Tainan 5. |
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