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| 題 名 | 生物檢定本土性蟲生線蟲(Steinernema taiwanensis Strain T39)感染昆蟲寄主及其人工繁殖之初探=Bioassay on the Infectivity of the Endogenous Entomopathogenic Nematode, Steinernema taiwanensis Strain T39, against Insects and Its Mass Production |
|---|---|
| 作 者 | 曾慶慈; 侯豐男; 唐立正; | 書刊名 | 臺灣昆蟲 |
| 卷 期 | 37:1 2017.03[民106.03] |
| 頁 次 | 頁11-22 |
| 分類號 | 433.3 |
| 關鍵詞 | 蟲生線蟲; 感染力; 生物檢定; 人工培養; Entomopathogenic nematode; Infectivity; Bioassay; Mass production; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 本試驗以生物檢定及不同培養法研究台灣本地產蟲生線蟲 (Steinernema taiwanensis strain T39) 對昆蟲寄主之殺蟲效力及人工飼育量產上之應用潛能。分別以一對一檢定法接種斜紋夜蛾 (Spodoptera litura Fabricius) 或大蠟蛾 (Galleria mellonella Linnaeus) 顯示,不同培養法所 得線蟲造成之寄主死亡率彼此間無顯著差異;若同樣接種體外培養線蟲,兩種寄主之死亡率亦無顯 著差異。以 S. taiwanensis strain T39 接種昆蟲寄主進行活體培養時,平均每公克之斜紋夜蛾六 齡幼蟲蟲體能產出 7.36×103 IJs (infective juveniles),大蠟蛾末齡幼蟲則為 2.00×104 IJs,此兩 結果彼此具顯著差異,顯示大蠟蛾較斜紋夜蛾更適於作為 S. taiwanensis strain T39 之活體培養 寄主。在檢測共生菌之菌體量時發現,培養 48 h 之菌液 OD550nm為 0.49 較 72 h 菌液之 0.52 為低, 然其於 NBTA 平板上生長之菌落數較多,顯示培養 48 h 之菌液內含活菌數較 72 h 為高。藉由固 態及液態人工培養法測試 S. taiwanensis strain T39,固態培養之產量約為 3.69×105 IJs/mL, 液態培養則約為 1.81×105 IJs/mL。以不同體外培養方式所得之 S. taiwanensis strain T39 懸浮 液接種斜紋夜蛾五齡幼蟲,接種20 IJs/mL時,液態培養所得之LT50為28.25 h較固態培養之40.71 h 短,而其 72 h 之累積死亡率亦相對較高,然彼此間並無顯著差異,顯示兩種人工培養法所得之 線蟲雖於致病速率上有所差異,但皆能有效殺死昆蟲寄主。綜合上述結果證實對昆蟲寄主具感染力; 另外,此線蟲可用斜紋夜蛾及大蠟蛾生產,亦能以體外培養法進行人工飼育量產,而所得之後代線 蟲仍具有殺蟲效力。 |
| 英文摘要 | Infectivity of the entomopathogenic nematode, Steinernema taiwanensis strain T39, isolated from Taiwan was investigated using a one-on-one assay against two insect hosts, Spodoptera litura and Galleria mellonella, and its mass production was carried out by in vivo and in vitro culture methods in this study. The inoculation of S. litura and G. mellonella larvae with the in vivo and in vitro cultures of infective juveniles (IJs) revealed no significant differences in cumulative mortalities among various treatments. The average yield of S. taiwanensis was lower when cultured with S. litura sixth instar larvae than with G. mellonella (ca. 7.36×103 vs 2.00×104 IJs/g), indicating that G. mellonella is more suitable for S. taiwanensis production. The OD550nm values of symbiotic bacteria cultured for 48 and 72 h in nutrient broth were 0.49 and 0.52, respectively. However, the number of bacterial colonies cultured in nutrient bromothymol blue agar for 48 h was significantly higher, indicating that the viability of the 48-h culture was higher than that of the 72-h culture. The solid and liquid cultures of S. taiwanensis yielded an average of 3.69×105 and 1.81×105 IJs/mL, respectively. The median lethal time (LT50) values of S. litura fifth instar larvae inoculated with 20 IJs/mL of S. taiwanensis produced in the liquid and solid cultures were 28.25 and 40.71 h, respectively. Although the cumulative mortality of the larvae inoculated with liquid-cultured nematodes was slightly higher, it was not significantly different from that of the larvae inoculated with solid-cultured nematodes. Therefore, nematodes produced by both in vitro methods differed only in the time to kill insects. In conclusion, S. taiwanensis exhibits infectivity against insects and can be cultured artificially to produce virulent offspring. |
本系統中英文摘要資訊取自各篇刊載內容。