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題 名 | Capability of Neurite Regeneration of Rat Retinal Explant at Different Ages=不同年齡之大白鼠視網膜人工培養的神經軸突再生能力的比較 |
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作 者 | 蔡榮坤; 許明木; 王惠珠; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 14:4 1998.04[民87.04] |
頁 次 | 頁192-196 |
分類號 | 416.746 |
關鍵詞 | 大白鼠; 視網膜; 人工培養; 神經軸突再生能力; Retinal explant; Neurite regeneration; Rat development; |
語 文 | 英文(English) |
中文摘要 | 剛出生的哺乳動物之中樞神經組織在體外培養具有神經軸突再生的能力,但是成人期的中樞神經組織就喪失此能力。為了解答大白鼠的視網膜人工培養在出生後多久就會喪失軸突再生的能力,我們利用出生後不同年齡的大白鼠之視網膜進行組織培養。出生後第一天,第三天,一個星期,二個星期,三個星期,四個星期及成人大白鼠之視網膜於組織培養 96 小時後比較神經軸突生長的情形。利用免疫化學染色,這些生長的神經軸突可被對抗神經細絲的單株抗體所染色。實驗結果顯示大白鼠的視網膜於組織培養中隨著年齡的增加而逐漸遞減其神經軸突的再生能力,而在出生後兩星期後完全喪失此功能。此外,大白鼠視網膜在人工培養中喪失神經軸突再生能力的年齡與大白鼠眼睛睜開及視網膜成熟的時間相吻合(出生後第15至18天,平均16.2 ±1.3天)。本結果顯示出當視網膜發育成熟時,就喪失了在體外培養之神經軸突再生的功能。 |
英文摘要 | Neurite regeneration from central nervous tissue of mammalian has been demonstrated to occur in early postnatal stage but not in adult stage. To answer when the retInal explant of rat loses the neurite regeneration in vitro after birth, we have used tissue culture to study the neurite regeneration of retinal explant of Wistar rat at different ages. The retinal explants from rat at first postnatal day, 3rd postnatal day, one week, two weeks, three weeks, four weeks old and of the adult were putin tissue culture to observe the neurite growth after four-day incubation. The neurites were also Identified by immunochemical staining using monoclonal antibody to neurofilament. The capabilityof neurlte regeneration of retinal explant was decreased with the passage of time after birth, and it was lost after two weeks old. The age of losing retinal regenerative capability coincides with that of eye opening and retinal maturation of Wistar rat (l5th-l8th postnatal day, mean 16.2 ± 1.3 days). These results indicate that when the development of retina has been completed, it will lose the capability of regeneration in vitro. |
本系統中英文摘要資訊取自各篇刊載內容。