查詢結果分析
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頁籤選單縮合
題名 | Detection of Loci Affecting Body Weight and Body Conformation Measurements in Nubian Goat Using Microsatellite Markers=利用微衛星標識檢測影響努比亞山羊體重及體型性狀之基因座 |
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作者 | 凃柏安; 苗雨蒔; 賴芳裕; 張俊達; 楊深玄; 王佩華; | 書刊名 | 中國畜牧學會會誌 |
卷期 | 45:1 2016.03[民105.03] |
頁次 | 頁71-88 |
分類號 | 437.58 |
關鍵詞 | 體型測定; 遺傳多樣性; 生長性狀; 標識輔助選拔; Body conformation measurement; Genetic diversity; Growth traits; Marker-assisted selection; |
語文 | 英文(English) |
中文摘要 | 畜牧產業非常重視動物的生長表現,動物的生長表現同時影響市場行銷與飼養成本。標識輔助選拔( marker-assisted selection, MAS)則可提高選拔準確度及選拔效率,因此尋找並驗證基因標誌與目標性狀的關聯性對於建立 MAS系統十分重要。本研究的目的為確認努比亞山羊的生長及體型性狀與相關微衛星標識附近之基因片段之關聯性,以及確認與這些性狀相關連的染色體區域。試驗利用 15組微衛星標識組進行 109頭努比亞山羊的基因型分析,並進一步分析各衛星標識基因型與出生、離乳以及 6月齡的體重及日增重;離乳至 6月齡的體長、體高、胸圍等體型性狀相關性。數量性狀基因座( quantitative trait loci, QTL)及候選片段之分析,則是利用最小平方回歸區間定位法( least squared regression interval mapping)進行分析。試驗結果顯示,共有 11個可能的 QTL達到顯著標準,分別位於第 2、3、5、7、13、14、15、18、20、21 及 23號染色體上,其中位於第 5、13、14、18、20及 23號染色體上,分別有 6個與不同生長時期體重相關的 QTL。不同時期日增重相關之 QTL則分別位於第 3、5、14、15、18及 20號染色體上。至於體型性狀相關的 QTL,共有 6個與不同時期體長性狀相關的 QTL,分別位於第 2、5、14、18、20及 21號染色體上; 7個與不同時期體高性狀相關的 QTL分別位於第 3、5、7、15、 18、20及 21染色體上; 3個與不同時期胸圍性狀相關的 QTL分別位於第 7、 14及 18號染色體。其中部分與體重、日增重及體型性狀的 QTL位置可以對應到其他相近之物種如綿羊及牛,顯示這些染色體區段可能存在有影響努比亞山羊體重、日增重及體型性狀的基因座。 |
英文摘要 | Growth performance of animal is always of great concern in livestock industry. It directly influences marketing age and the feeding cost of animals. Marker-assisted selection (MAS) is a powerful tool with accuracy and efficiency. Seeking and validating genetic markers of target traits are crucial for establishing a MAS system. The objective of this study was to determine whether there is evidence for association of growth traits and body conformation measurements in Nubian goats in Taiwan with markers flanking specific chromosome regions, and to identify chromosomal regions associated with various growth traits. Fifteen microsatellite markers were used for genotyping 109 Nubian goats, and further analyzed their relationships with body weight and average daily gain (ADG) at birth, weaning, and 6 month of age, respectively. The body conformation measurements including body length, withers height, and chest girth at weaning and 6 month of age were also analyzed. Quantitative trait locus (QTL) analysis in the candidate intervals was conducted using the least squared regression interval mapping approach. A total number of 11 putative QTL at chromosome-wide significance level were detected on chromosome 2, 3, 5, 7, 13, 14, 15, 18, 20, 21, and 23 . Six putative QTL were identified for body weight at different ages on Capra hircus chromosome (CHI) 5, 13, 14, 18, 20, and 23. And 6 putative QTL were identified for ADG at different ages on CHI 3, 5, 14 , 15, 18, and 20. With regard to the body conformation measurements, 6 putative QTL were identified for body length at different ages on CHI 2, 5, 14, 18, 20, and 21 ; seven putative QTL were identified for withers height at different ages on CHI 3, 5, 7, 15, 18, 20, and 21 ; three putative QTL were identified for chest girth at different ages on CHI 7, 14, and 18. Some of these QTL correspond to chromosomes where QTL associated with body weight, ADG, and body conformation measurement traits have been identified in other closely related species such as sheep and cattle. These chromosomal segments may contain candidate genes that potentially influence body weight, ADG, and body conformation measurements in Nubian goats. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。