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題名 | Production of Potato Protein Hydrolysates with Lipolysis-stimulating Activity and Study of Their Possible Regulation Mechanism=生產具有促脂解活性的馬鈴薯蛋白水解物與研究其可能的調節機制 |
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作者 | 林万登; 李貴宜; 洪敏純; 江文德; | 書刊名 | 臺灣農業化學與食品科學 |
卷期 | 54:2 2016.04[民105.04] |
頁次 | 頁63-73 |
分類號 | 463.1 |
關鍵詞 | 馬鈴薯蛋白水解物; 脂解作用; 脂肪細胞; 三酸甘油酯; 濾膜區分作用; Potato protein hydrolysate; Lipolysis; Adipocyte; Triglyceride; Membrane fractionation; |
語文 | 英文(English) |
中文摘要 | 本研究目的在利用3T3-L1脂肪細胞探討馬鈴薯蛋白水解物(PPH)的促脂解活性;胞內相對脂肪殘留量(RTR)被用來作為細胞脂解作用的指標,RTR愈低代表有較佳的促脂解活性。在六種酵素中以Alcalase水解馬鈴薯蛋白(PP)6小時所得水解物APPH6h具有最低的RTR。其次APPH6h利用30-1千道爾吞(kilo-daltons; kDa)分子量限值(Molecular weight cut-off; MWCO)濾膜進行序列區分,所獲得的10 kDa MWCO保留液(retentate)可進一步提升APPH6h在細胞中的促脂解活性,當10 kDa MWCO retentate使用濃度為800 ppm時其RTR顯著的從88.4%降低至83.8%。腸胃道蛋白酶對於800 ppm 10 kDa MWCO retentate的促脂解活性沒有顯著的影響( p < 0.05)。根據西方轉漬分析及免疫染色分析的結果指出,10 kDa MWCO retentate在脂肪細胞中藉由荷爾蒙敏感性脂解酶的磷酸化及轉位提升其促脂解活性。 |
英文摘要 | The aim of this study was to explore the lipolysis-stimulating activity of potato protein hydrolysate (PPH) using 3T3-L1 adipocytes. Intracellular relative triglyceride residue (RTR) was employed as a marker for lipolysis in the adipocytes. The lower RTR represents the better lipolysis-stimulating activity. Among 6 enzymes, Alcalase was selected to hydrolyze potato protein (PP) for 6 h to obtain APPH6h with the lowest RTR. The sequential fractionation of APPH6h with 30-1 kDa molecular weight cut-off (MWCO) membranes in order to obtain a 10 kDa retentate resulted in further enhancement of its lipolysis-stimulating activity in the cells. The RTR significantly decreased from 88.4 to 83.8% at the 800 ppm level. Gastrointestinal proteases have no significant effect ( p > 0.05) on the lipolysis-stimulating activity of 10 kDa retentate at 800 ppm level. Based on Western blot analysis and immunostaining assay, 10 kDa retentate promotes lipolysis by phosphorylation and translocation of hormone-sensitive lipase (HSL) in the adipocytes. |
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