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題名 | Studies on Optimization of Enzymatic Hydrolysis Conditions for Soy Protein Hydrolysate with Triacylglycerols Accumulation Inhibitory Activity in 3T3-L1 Adipocytes=大豆蛋白水解物在3T3-L1脂肪細胞中具有抑制三酸甘油酯堆積活性之最適化水解條件探討 |
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作者 | 盧錫祺; 鄒梅君; 高豪駿; 江文德; | 書刊名 | 臺灣農業化學與食品科學 |
卷期 | 50:5/6 2012.10-12[民101.10-12] |
頁次 | 頁249-254 |
分類號 | 346.77 |
關鍵詞 | 大豆分離蛋白; 三酸甘油酯堆積; 3T3-L1脂肪細胞; 反應曲面法; Soy protein isolate; Triacylglycerols accumulation; 3T3-L1 adipocytes; Response surface methodology; |
語文 | 英文(English) |
中文摘要 | 藉由反應曲面法(response surface methodology; RSM)探討Flavourzyme最適水解大豆分離蛋白(SPI)的條件,所得水解物在已分化的3T3-L1脂肪細胞中具有抑制三酸甘油酯(triacylglycerols accumulation; TA)堆積活性。本研究應用三變數五階層的中心混層試驗設計,這些獨立變數的範圍是pH5.32-8.68、反應溫度(reaction temperature; RT)33.2-66.8℃及水解時間(hydrolysis time; HT)19.2-220.8min。根據反應曲面和等高線圖的結果顯示,以Flavourzyme最適水解SPI所得水解物使細胞內的TA最小化的條件包括:pH=7.41, RT=49.48℃以及HT=140.63min。此結果也表示以二次式來描述反應曲面是合宜的,因為缺適性(lack of fit)分析所得之F值不顯著(p>0.05)。除此之外,此二次式有合理的R2(=0.904)係數,且經試驗證實。 |
英文摘要 | The optimal hydrolysis conditions of soy protein isolate (SPI) with Flavourzyme® for triacylglycerols accumulation (TA) inhibitory activity in differentiated 3T3-L1 adipocytes were investigated by a response surface methodology (RSM). A central composite rotatable design for three variables at five levels was applied in this study. The independent variables were pH 5.32-8.68, reaction temperature (RT) 33.2-66.8 t and hydrolysis time (HT) 19.2-220.8 min. According to the results of response surface and contour plots, the optimal hydrolysis of SPI with Flavourzyme® for minimizing the TA in the cells were: pH=7.41, RT=49.48℃ and HT 140.63 min. The results also implied that the second order model was appropriate for description of the response surface since the F-value for lack of fit was not significant (p>0.05). In addition, the model had a satisfactory coefficient of R2 (=0.904) and was verified experimentally. |
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