查詢結果分析
來源資料
頁籤選單縮合
題名 | 臺灣油杉人工授粉對種子生產效應與葉綠體DNA遺傳=Effects of Cross Pollination on Seed Production and the Inheritance of Chloroplast DNA in Keteleeria davidiana var. formosana |
---|---|
作者姓名(中文) | 鍾振德; 洪西洲; 簡慶德; | 書刊名 | 臺灣林業科學 |
卷期 | 31:3 2016.09[民105.09] |
頁次 | 頁181-197 |
分類號 | 436.251 |
關鍵詞 | 臺灣油杉; 天然授粉; 控制授粉; 輔助授粉; 有效種子; 葉綠體DNA; Keteleeria davidana var. formosana; Natural pollination; Controlled pollination; Supplemental mass pollination; Seed efficiency; Chloroplast DNA; |
語文 | 中文(Chinese) |
中文摘要 | 台灣油杉僅分布於台灣本島之南北兩端,族群稀少,結實率低,已被列為積極保育之固有樹種。本研究於2002~2010年間,在7個地點共採集10株母樹之天然授粉毬果,包括人工栽植之台北植物園、台北麗水街、陽明山、羅東出水溪、福山植物園,及坪林金瓜寮與石槽等2處原生地。台北植物園飽滿種子率低於1%,而麗水街與陽明山兩地為獨立木,飽滿種子來自自花授粉,有效種子率0.03~3.4%,但在2004年兩株有效種子率卻分別為10.1與7.1%。2009年坪林金瓜寮與石槽,有效種子率分別為2.8與19.1%。台灣油杉豐歉年沒有一定規律性,一般3~5年1次豐年,豐年過後翌年是歉年,不同地點豐歉年的毬果數量差異很大。人工控制授粉試驗,以陽明山與台東達仁種源母樹為花粉源,自花授粉毬果之有效種子率為0.08%,異花授粉毬果之有效種子率為14.7~37.9%。採集自坪林與達仁母樹之新鮮花粉含水率約14.5%,花粉發芽率71~95%,經真空冷凍乾燥後之花粉發芽率53~96%。此乾燥之花粉儲藏在-20℃經5年後進行輔助授粉試驗,結果顯示每毬果的有效種子率可達5.0~48.2%,遠比天然授粉高。利用葉綠體DNA基因trnD-trnY與trnV intron區間序列定序分析,以控制授粉所得之後裔為材料,證實台灣油杉葉綠體DNA百分之百為父系遺傳。 |
英文摘要 | Keteleeria davidiana var. formosana is an indigenous tree native only to the northern Pinglin and southern Dawu areas of Taiwan. It is listed as a protected species by the Taiwanese government due to the scarcity of its populations and low seed production. Cones of 10 naturally pollinated Keteleeria mother trees were collected from the Taipei Botanical Garden, Taipei's Lishui Street, Yangmingshan, Chushui River nursery, Fushan Botanical Garden, Pinglin's Jingualiao River, and Pinglin's Shicao in 2002~2010. The number of filled seeds to total number (filled+empty) of seeds per cone was consistently < 1% in the Taipei Botanical Garden. Single mother trees at Lishui Street and Yangmingshan were naturally self-pollinated, and seed efficiency per cone ranged 0.03~3.4% during the study period, except for cones collected in 2004 for which seed efficiencies per cone were 10.1 and 7.1%, respectively. The mother trees at Pinglin's Jingualiao River and Pinglin's Shicao were located in natural populations, and seed efficiencies per cone, which were determined only in 2009, were 2.8 and 19.1%, respectively. Cone production was irregular, with good masting every 3~5 yr. Cone production varied from 1 population to another during a good masting year, and it also differed within a population, i.e., some mother trees produced abundant cones but others did not. For controlled pollination, pollen of 1 genotype from Yangmingshan and 1 from southern Daren were used. Seed efficiencies per cone for both areas were 14.7~37.9% in controlled pollination compared to 0.08% for self-pollination. For supplemental mass pollination, dried pollen with 53~96% germination stored at -20℃ for 5 yr was used. Seed efficiencies per cone ranged 5.0~48.2%, which were greater than that of natural pollination. trnD-trnY and trnV intron intergenic spacer analyses showed that chloroplast DNA is paternally inherited. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。