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題名 | 臺灣油杉未熟胚之胚原癒合組織誘導與體胚發生=The Induction of Embryogenic Callus and Somatic Embryogenesis in Immature Zygote Embryo Cultures of Keteleeria Davidiana Var. Formosana |
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作者 | 張淑華; 何政坤; 陳怡蒨; 蔡錦瑩; | 書刊名 | 中華林學季刊 |
卷期 | 36:4=133 2003.12[民92.12] |
頁次 | 頁321-329 |
分類號 | 436.251 |
關鍵詞 | 臺灣油杉; 體胚發生; Keteleeria davidiana var. formsana; Somatic embryogenesis; |
語文 | 中文(Chinese) |
中文摘要 | 將正處於分裂性多胚的臺灣油杉(Keteleeria davidiana var. formsana)未熟種子,培養在含有不同濃度的2,4-D與BA組合的4種基本培養基中培養,結果在LP培養基添加5mg/L 2,4-D組合0或0.5mg/LBA與BLG培養基添加3mg/L 2.4-D可誘導出胚原性癒合組織。胚原性癒合組織可在暗處理的培養室快速增生,唯形態都屬stage 1 embryos。胚原性癒合組織的生長速率在含有與不含植物生長調節劑的LP培養基中並無顯著差異,而且該癒合組織可長期培養在不含植物生長調節劑的培養基仍生長良好,顯示該胚原性組織可能含有相當濃度的生長素。Stage 2 embryos可在光照環境下誘導出來,將此體胚培養在含有ABA與不含植物生長調節劑的培養基,均可誘導出具有4枚子葉的成熟體胚,移植個別的成熟體胚至基本培養基培養,大約5%的體胚可發芽成苗。 |
英文摘要 | Immature seeds of Keteleeria davidiana var. formsana, which were at cleavage embryo stage, were cultured on 4 basal media containing various concentrations of 2,4-D and BA. Embryogenic masses were induced on LP medium supplemented with 5 mg/L 2,4-D and 0 or 0.5 mg/L Ba and BLG medium containing 3 mg/L 2,4-D. They grew readily and maintained in stage 1 embryos in the dark culture room. The growth rates of embryogenic masses culturing on either plant growth regulator (PGR) containing or free media did not differ significantly. The masses could maintain in a good growth condition on PGR free medium for a long period. It presumes that embryogenic masses might contain enough auxin content to promote their growth on hormone free medium. Stage 2 embryos were induced when masses cultures were transferred to light condition. These embryos then developed into mature embryos with 4 cotyledons on LP medium with ABA or without any PGR. Of these mature embryos isolated from these cultures. Only less than 5% could germinate into emblings. |
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