查詢結果分析
來源資料
相關文獻
- 芒果切離葉接種技術評估生物防治菌防治炭疽病
- Germination and Appressorium Formation in Colletotrichum Gloeosporioides
- 芒果炭疽病菌分生孢子、發芽管及附著器胞外粘質之偵測及定性
- 芒果炭疽病之生物防治
- 影響芒果炭疽病菌附著器形成與發芽之因子
- 芒果炭疽病菌胞子發芽之營養需求及胞子外泌物之特性
- 臺灣芒果炭疽病菌胞子外泌物之酵素組成分析
- 臺灣芒果炭疽病菌之分群
- 利用PCR-RFLP調查臺南地區抗苯並咪唑類(benzimidazoles)殺菌劑芒果炭疽病菌的發生
- Evaluation of Fungicidal Resistance among Colletotrichum Gloeosporioides Isolates Causing Mango Anthracnose in Agri Export Zone of Andhra Pradesh, India
頁籤選單縮合
題 名 | 芒果切離葉接種技術評估生物防治菌防治炭疽病=Development of Detached Mango Leaf-inoculation System for Rapid Evaluating the Efficacy of Biocontrol Agent on Controlling Mango Anthracnose |
---|---|
作 者 | 劉冠霆; 邱國棟; 謝岱庚; 黃振文; 李敏惠; | 書刊名 | 植物病理學會刊 |
卷 期 | 24:3/4 2015.12[民104.12] |
頁 次 | 頁189-199 |
分類號 | 435.325 |
關鍵詞 | 芒果; 炭疽病菌; 葉齡; 葉表; 葉背; 角質層; 接種系統; 微生物製劑; Mango; Colletotrichum gloeosporioides; Leaf age; Adaxis; Abaxis; Cuticle layer; Inoculation system; Microbial biopesticides; |
語 文 | 中文(Chinese) |
中文摘要 | 炭疽病菌Colletotrichum gloeosporioides Penz.為芒果栽培之最重要病原菌,可以感染芒果樹的葉、花穗、枝條及採收前與採收後果實,並造成產量及品質嚴重受損。芒果樹每年僅結果或開花一次,若使用為接種材料作為病害防治策略之建立或資材之開發,相當不符合效率,芒果葉片亦為芒果炭疽病之感病器官,但不同葉齡對芒果炭疽病之感受性差異大,使得實驗結果不穩定,本研究以C. gloeosporioides TYC-2菌株作為探討炭疽病菌對芒果葉片侵染之影響因子,包括葉齡、葉表及葉背對炭疽病菌侵染之影響以建立穩定切離葉炭疽病接種系統。實驗首先測試C.gloeosporioides TYC-2 菌株對各種芒果品系之毒力及其適合生長溫度,實驗結果顯示TYC-2菌株對14種供試芒果果實大都具有一定致病能力,24-28℃為適合生長之溫度。以不同葉齡之切離葉進行接種,發現抽芽後8天內之愛文芒果葉片較為感病,以孢子噴霧接種可穩定發病,葉齡越小發病速度越快,接種後最快48小時即可見壞疽病徵。葉齡30天以上之成熟葉則需傷口處理後再接種才可產生炭疽病徵。以8天內葉齡葉片之葉表及葉背進行孢子懸浮液點狀接種,結果顯示葉背較葉表感病,葉背可於葉表接種後48小時出現細胞壞死褐化,而葉表則於72小時後才出現同樣病徵,觀察及計數孢子發芽及附著器形成情形,發現孢子發芽及附著器形成率與形成時間在葉表及葉背並無差異。進一步以切片配合角質層染色,發現成熟及未成熟葉之上表皮細胞層皆較下表皮細胞層厚,成熟芒果葉片之角質層染色呈色較未成熟葉明顯。利用此接種系統進行TYC-2 菌株之拮抗菌的不同發酵液之不同稀釋倍率對炭疽病之防治效果測試,顯示各重複間發病穩定,可看出各處理間的明顯差異。本切離葉接種系統以抽芽後8 天內之愛文芒果葉片進行500顆孢子的多點接種或10^5 spores/ml 之噴霧接種可獲得穩定之發病。本研究顯示此接種系統可提供微生物製劑研發使用。 |
英文摘要 | Mango is one of the most important fruit crops and the highest value of exported fresh fruits in Taiwan. Colletotrichum gloeosporioides causes mango anthracnose and is one of the major pathogens on mango. It can infect mango leaf, flower, branch and fruit, and causes considerable fruit disqualified during postharvest period. To develop disease control strategies or agents, a stable and readily available inoculation system is required. However, mango fruit and flower are not obtainable all seasons, and the susceptibility of mango leaf to C. gloeosporioides is notably affected by leaf age. In this study, C. gloeosporioides TYC-2 strain was used as the tested pathogen to investigate the factors, which might influence the infection of C. gloeosporioides on mango leaf, including leaf age and leaf surfaces, to setup a stable inoculation system using detached leaf. The virulence of C. gloeosporioides TYC-2 strain on various mango cultivars was verified and the optimal growth temperature of TYC-2 strain was measured. TYC-2 strain expressed high virulence to most of the assayed mango cultivars and had optimal growth temperature around 24-28℃. Leaves (cv. Irwin) with age less than 8-days were consistently susceptible to C. gloeosporioides TYC-2 after sprayed or spot inoculation with spore suspension. Younger leaf expressed higher susceptibility than older leaf. Necrotic lesion could be observed within 48 hs post-inoculation on the leaf younger than 6 days. In addition, the abaxial leaf surface is more susceptible than the adaxial leaf surface to C. gloeosporioides TYC-2. There was no difference on C. gloeosporioides germination and appressorium formation between the adaxial and abaxial surface of a leaf. The cuticle and epidermis of the adaxis was thicker than the abaxis of the leaf, indicating the thickness of cuticle layer might be a key factor for the resistance of mango leaf to C. gloeosporioides TYC-2. Using the detached leaf-inoculation system, we tested the disease control efficacy of two different fermentation liquids with various dilution factors, which were collected from a bacterial antagonist of TYC2 strain after cultured in two different fermentation media. The results showed stable, repeatable and significant differences on mango anthracnose disease control between the two tested fermentation liquids. In this detached leaf-inoculation system, leaves (cv. Irwin) with age less than 8 days inoculated with C. gloeosporioides by spraying (105 spores/ml) or multiple drops (500 spores/10 μl) inoculation resulted in consistent and stable disease development. This inoculation system was demonstrated to be capable of using in mango anthracnose disease control for the study and development of microbial biopesticides. |
本系統中英文摘要資訊取自各篇刊載內容。