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頁籤選單縮合
題 名 | 影響芒果炭疽病菌附著器形成與發芽之因子=Factors Affecting Appressorium Formation and Germination of Mango Anthracnose Fungus |
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作 者 | 莊再揚; | 書刊名 | 植物病理學會刊 |
卷 期 | 6:2 1997.06[民86.06] |
頁 次 | 頁41-48 |
分類號 | 435.325 |
關鍵詞 | 芒果炭疽病; 炭疽病菌; 附著器; Mango anthracnose; Colletotrichum gloeosporioides; Appressorium; |
語 文 | 中文(Chinese) |
中文摘要 | 利用玻璃紙方法探討營養、相對濕度、溫度、不同菌系及胞子濃度對芒果炭疽病菌附著器形成的影響,同時亦觀察營養及附著器乾燥時間對附著器發芽的影影。在葡萄糖濃度0-1000ppm範圍內,三株芒果炭疽病菌胞子發芽並不受影響,但各菌株胞子發芽卻顯著不同。相同的葡萄糖濃度亦不影響已發芽胞子形成附著器,而附著器形成能力亦視菌株不同而異。在相對濕度100%與98%時,附著器形成率並無顯著差異,以後隨著相對濕度下降,附著器形成率顯著減少,而在相對濕度90%時,胞子並不發芽,亦無附著器形成。在飽和相對濕度下,20C可佔進已發芽胞子形成附著器,但在28C或28C/20C各12小時的交互溫度條件下,均不利於附著器的形成,而16C會延緩附著器的形成。各菌株形成附著器能力差異很大,且會受胞子濃度影響。在供試的20個菌株中,僅有4個菌株由已發芽胞子形成附著器比例高達70-80%但卻有10個菌株附著器形成率低於50%。乾燥後儲存2週的附著器,泡浸於1%glucose-0.1% aspartic acid混合營養液中,其發芽率較在water agar、potato dextrose agar (PDA)或先浸在營養液2小時再移植於PDA者為高,且各菌株附著器發芽率的差異很大。附著器在1% glucose-0.1% aspartic acid混合營養液泡浸12小時後開始發芽,30小時後,發芽達到高峰。含附著器的玻璃紙經乾燥後儲放在25C下,在第一週附著器並不發芽,第二週開始發芽,而在第六週發芽達到高峰,以後發芽率逐漸下降。 |
英文摘要 | Effect of nutrient, relative humidity (RH), temperature, fungal strains and spore concentration on appressorium formation of Collectorichum gloeosporioides was investigated by using cellophane system in this study. In addition, the effect of nutrient and storage of dried appressoria formed on cellophane on the apprssorium germination of the fungus was also determined. Among three isolates of C. gloeoporioides tested, condidial germination as well as appressorium formation were not affected at glucose concentration ranging from 0 to 1000 ppm. However, condial germination and appressorium formation varied with isolates tested at the same range of the glucose concentration. The appressorium formation was not significantly different at RH 10% and 98%. And then appressorium formation was decreased with decreasing RH significantly. Niether condial germination nor appressorium formation was observer at RH 90%. Appressorium formation from germinating spores was enhaced at 20 C, however, it was not favored for appressorium formation at 28 C and 28 C/20 C for 12 hr alternate temperature. While appressorium formation might be delayed at 16 C. The ability for appressorium formation was significantly by isolates tested as well as spore concentration. Among 20 isolates tested, only 4 isolates showed appressorium formation higher than 70%, however, 10 isolates whoed less than 50%. Dried appressoria formed on cellophane stored at 25 C for 2 weeks wee tested for appressirum germination. The appressoirum germination. The appressorium germination was higher when the cellophanes were soaked in 1% glucose-0.1% aspartic acid nutrient solution, compared to placing the cellophanes on water agar or PDA directly or soaking in the nutrient solution for 2 hr and then placed on PDA. Appressorium germination was started 12 hr after soaking in the nutrient solution, and reached to a maximum after 30 hr incubation. Appressorum germination began at the 2nd week and reached to a maximum at the 6th week, when the dried appressoria formed on cellophane were stored at 25 C. |
本系統中英文摘要資訊取自各篇刊載內容。