查詢結果分析
來源資料
相關文獻
- 利用馬兜鈴酸腎鉦腎炎模型評估綠茶(+)-catechin 的藥效
- 馬兜鈴酸腎症在純系小鼠的確立與柴胡在此腎炎模型的藥效評估
- 馬兜鈴酸腎症在純系小鼠的確立與柴胡在此腎炎模型的藥效評估
- 人參及人參皂苷於純系小鼠之馬兜鈴酸腎炎模型的藥效評估
- 粗製巴斯德桿菌D型毒素誘發腫瘤壞死因子-α產生與豬萎縮性鼻炎關係之探討
- Density Heterogeneity of Alveolar Macrophages: Induction of Hypodense Alveolar Macrophages by Respiratory Burst Activation
- Detection of Tumor Necrosis Factor-α and Receptors in the Serum and Synovial Fluid of Patients With Rheumatoid Arthritis and Osteoarthritis
- 研磨綠茶粉的製造方法與貯藏
- 年齡、豬生殖與呼吸綜合症病毒及豬霍亂沙氏桿菌感染對豬肺泡巨噬細胞的表現型和功能之影響
- The Processing of Metabolized Low Density Lipoproteins in Foam Cells Generates Superoxide Anions
頁籤選單縮合
題 名 | 利用馬兜鈴酸腎鉦腎炎模型評估綠茶(+)-catechin 的藥效=Effect of Green Tea and (+)-Catechin on Aristolochic Acid-induced Nephropathy in Inbred Mice |
---|---|
作 者 | 陳世銘; 許光陽; 許光陽; | 書刊名 | 中醫藥年報 |
卷 期 | 3 2014.12[民103.12] |
頁 次 | 頁(46)1-(46)59 |
分類號 | 414.5 |
關鍵詞 | 馬兜鈴酸腎病變; 綠茶; 巨噬細胞; 乙型轉型生長因子; 基質金屬蛋白分解酶; 甲型腫瘤壞死因子; (+)-catechin; Aristolochic acid nephropathy; AAN; Green tea; Macrophage; TGF-β; Transforming growth factor-β; MMP-9; Matrix metalloproteinase-9; TNF-α; Tumor necrosis factor-α; |
語 文 | 中文(Chinese) |
中文摘要 | 研究目的:近年來發生許多馬兜鈴酸腎病變 (aristolochic acid nephropathy, AAN)的案例,其原因是誤用含馬兜鈴酸的植物。腎臟受損部位侷限於近曲小管,有明顯的細胞浸潤、腎小管萎縮,以及間質纖維化的情形。本研究藉由長期投予純系小鼠低劑量 aristolochic acid (AA)成功誘發與人類 AAN相似的腎炎,並評估綠茶 (green tea, GT)、(+)-catechin (CAT)對 AAN的改善效果。 研究方法:純系小鼠 C3H/He (6weeks, males) 連續 56天飲水給予 3.0 μg/mL AA,之後停藥恢復正常飲用水觀察 14天;對照組在實驗期間投予正常飲用水。藥效評估實驗依前述方法給予 56天 AA後,連續 14天經口投予 GT 75, 150, 300 mg/kg, CAT 25, 50, 100 mg/kg對照組則給予蒸餾水。測定尿蛋白, N-acetyl-beta-D-glucosaminidase (NAG)與血中尿素氮 (blood urea nitrogen, BUN),來評估小鼠腎功能;腎組織使用 PAS染色觀察病理組織改變,並進行免疫螢光染色,包括:巨噬細胞,乙型轉型生長因子 (TGF-β),基質金屬蛋白分解酶(MMP-9),甲型腫瘤壞死因子 (TNF-α),以辨識損傷部位之特異性抗原。 結果與討論:實驗結果顯示,藉由飲用水連續給予 56天 AA會造成尿蛋白、 NAG、BUN及血糖值輕微上昇;腎組織鏡檢發現腎小管萎縮、間質有細胞浸潤和纖維化的情形,免疫螢光染色發現腎小管間質巨噬細胞浸潤、 TGF-β、TNF-α沉積明顯增加, MMP-9也有些許沉積,此外,腎臟受損的情形在停止投予 AA後不但沒有恢復,反而惡化。在投予 GT 150 mg/kg,CAT 50 mg/kg之尿蛋白 (GT 150 mg/kg除外)、 NAG、BUN、血糖值都有降低;組織學觀察發現腎小管萎縮、間質細胞浸潤和纖維化的情形皆有緩解,皆會改善 AAN病人腎臟功能。 |
英文摘要 | AIM: Many cases about aristolochic acid nephropathy (AAN) have been identified in recent years. The reason is that people mistaken the herb that contains aristolochic acid (AA). AAN is a unique type of nephropathy, which was characterized by extensive cell infiltration, tubular atrophy, and interstitial fibrosis. We established a chronic interstitial fibrosis model of AAN in inbred mice and investigated the efficacy of green tea (GT), (+)-catechin (CAT) on AAN. METHOD: AA was dissolved in distilled water (3μg/mL) and as drinking water ad libitum to C3H/He mice (6 weeks, male) for 56 days, the control group was administered to distilled water. In the second experiment, they were administered orally with GT (75, 150, 300 mg/kg), CAT (25, 50, 100 mg/kg) once daily for 14 days. The control group was administered to distilled water. Urinary protein (UP), urinary N-acetyl-beta-D-glucosaminidase (NAG), blood urea nitrogen (BUN), blood glucose were determined. Renal tissues were served to histological examination (PAS stain and immunofluorescence staining). The antibodies, including murine F4/80 macrophage, TGF-β (transforming growth factor-β), MMP-9 (matrix metalloproteinase-9), TNF-α (tumor necrosis factor-α) were chosen to recognize the specific antigens, which deposited in injury sites. RESULTS & DISCUSSION: All animals treated with AA developed increased in UP, NAG, BUN, and blood glucose. In the histological examination, we observed tubular atrophy, interstitial infiltration and fibrosis in the typical AAN. In the immunofluorescence stain assay, macrophage, TGF-β, MMP-9, and TNF-α were localized in the renal tissue. After discontinuation of AA, renal function did not improve. The amount of urinary protein, NAG, BUN, and blood sugar were decreased in the GT 150 mg/kg, CAT 50 mg/kg -treated groups compared with the control group (except for the UP of GT 150 mg/kg ). In the histological examination, we observed the alleviation in all treatment groups. The staining intensity of macrophage, TGF-β, TNF-α were decreased in all groups, and MMP-9 in the interstitium was significantly increased. Our results demonstrate the therapeutics efficacy of the treatment can improve the renal function of AAN patients. |
本系統中英文摘要資訊取自各篇刊載內容。