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頁籤選單縮合
題名 | 中西藥交互作用體內-體外試驗模型之建立及相關性探討(2-2)=Establishment and Evaluation of an in Vivo-in Vitro Model for Herb-drug Interaction (2-2) |
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作者姓名(中文) | 李珮端; |
作者姓名(外文) | Lee Chao, Pei-dawn; |
書刊名 | 中醫藥年報 |
卷期 | 光碟版1:3 2012.10[民101.10] |
頁次 | 頁372-404 |
分類號 | 418.1 |
語文 | chi |
關鍵詞 | 甘草; 甘草酸; 中西藥交互作用; P-醣蛋白; 多重耐藥性蛋白; Licorice; Glycyrrhizin; Herb-drug interaction; P-glycoprotein; Multidrug resistance proteins; |
中文摘要 | 研究目的: 國人的特殊用藥習慣,隱藏著中西藥發生交互作用的風險。目前可供快速篩檢中西藥交互作用的簡易模型仍極有限,本計畫以治療指數狹窄的 cyclosporine (CSP)與 methotrexate (MTX)為西藥模型藥物,分別代表 P-glycoprotein (P-gp)與 multidrug resistance proteins (MRPs)之受質,以甘草為中藥模型藥物,建立中西藥交互作用之簡易篩檢模型。 研究方法: 本計畫第二年係基於第一年體內試驗的結果,建立適宜的體外試驗模型。以人類大腸癌細胞株 LS 180探討甘草酸、甘草次酸、甘草水煎劑及芍藥甘草湯對 P-gp與 MRP2受質運輸及基因表現的影響。 結果與討論: 本體外試驗結果顯示,甘草酸、甘草次酸、甘草水煎劑及芍藥甘草湯皆顯著減少 P-gp受質在細胞中的蓄積,顯示它們皆為 P-gp的誘導劑;而甘草酸、甘草次酸及高濃度的芍藥甘草湯皆顯著增加 MRP2受質在細胞中的蓄積,顯示其為 MRP2的抑制劑,此些現象皆與體內動力學交互作用的結果相符。因此,本計畫所建立的體外細胞模型,應可用於篩檢中藥與 P-gp或 MRP受質西藥之交互作用。 |
英文摘要 | The special medication habit of Taiwanese may result in potential risks of the herb-drug interaction. Till now, there was limited in vitro model available for fast screening of herb-drug interaction. In this study, licorice was used as a model drug of Chinese herb. Cyclosporine (CSP) and methotrexate (MTX) were used as model drugs for substrates of P-glycoprotein (P-gp) and multidrug resistance proteins (MRPs), respectively. By correlating in vivo and in vitro results, we established a fast in vitro screening model for herb-drug interaction. Based on the findings of in vivo pharmacokinetic interactions in the first year, LS 180 was used to investigate the influence of glycyrrhizin (GZ), glycyrrhetic acid (GA), licorice decoction (LD) and Shaoyao Gancao Tang (SYGCT) on the transport of fluorescent substrates and gene expression of P-gp and MRP2. The in vitro results showed that GZ, GA, LD and SYGCT significantly decreased the intracellular accumulation of P-gp substrate and thus were the inducers of P-gp. In addition, GZ, GA and high concentration of SYGCT significantly increased the intracellular accumulation of MRP2 substrate and thus were the inhibitors of MRP2. These in vitro results were consistent with the in vivo observations. Therefore, these in vitro models established in this study can be used for fast screening for the interaction of Chinese herbs with P-gp or MRP substrates. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。