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題名 | 液相層析串聯質譜儀檢測杭菊農藥殘留=Simultaneous Determination of Pesticides Residues in Chrysanthemum Flowers Samples by Liquid Chromatography-tandem Mass Spectrometry |
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作者 | 陳裕文; 巫嘉昌; 侯金日; | 書刊名 | 臺灣茶業研究彙報 |
卷期 | 33 2014.11[民103.11] |
頁次 | 頁65-94 |
分類號 | 343.8 |
關鍵詞 | 杭菊; 液-液萃取法; 液相層析串聯質譜儀; 多重農藥殘留; Chrysanthemum flower; Liquid-liquid extraction; LC/MS/MS; Multiple pesticide residues; |
語文 | 中文(Chinese) |
中文摘要 | 杭菊 (Chrysanthemum morifolium Ramat) 是一種常見的中藥材和保健植物,但是 近年來,杭菊中農藥超標的案例層出不窮,為了確保杭菊之取食安全,需發展出一 套簡單、快速、準確且有效的杭菊多重農藥殘留分析方法。本研究以液-液萃取法 (liquid-liquid extraction, LLE)、固相萃取法 (solid phase extraction, SPE) 進行樣品前 處理,並採用液相層析串聯質譜儀 (liquid chromatography-tandem mass spectrometry, LC/MS/MS) 建立杭菊乾花中 130 種多重農藥殘留分析方法。結果顯示,測試農藥 皆具有良好的線性關係 (r2 ≧ 0.995)。在最低檢測極限 (limit of detection, LOD) 和最 低定量極限 (limit of quantification, LOQ) 部分,有 97.69% 的農藥在 LC/MS/MS 儀器中測定的 LOD ≦ 10 μg/kg;有 85.38% 的農藥之 LOQ 為 10 μg/kg。在添加 10、20、50 和 100 μg/kg 四種不同濃度的農藥處理,130 種農藥中有 80-91.54% 的 農藥回收率介於 70-120% 且相對標準偏差 (relative standard deviation, RSD) < 20% 。本研究所建立之分析方法,分析效果良好,可應用在杭菊農藥微量殘留分析。 |
英文摘要 | Chrysanthemum morifolium Ramat is a common Chinese herbal medicine and healthy crops. In recent years, events of having exceeded maximum residue levels (MRLs) of pesticides in Chrysanthemum morifolium Ramat had been revealed one after another. Thus, it is necessary to develop a simple, quick, effective and rugged method for determining multiple pesticide residues in Chrysanthemum morifolium to ensure the safety of Chrysanthemum morifolium cultivation. In this study, we used liquid-liquid extraction (LLE) and solid phase extraction (SPE) as the sample preparation, followed by liquid chromatography-tandem mass spectrometry (LC/MS/MS) to develop four multi-residue methods for the determination of pesticides in Chrysanthemum morifolium. We developed a multi-residue method for the determination of 130 pesticides in dry chrysanthemum flowers by LLE with acetonitrile, amino cartridges for SPE and LC/MS/MS. The present results showed good linearity by correlation coefficients of r2 ≧ 0.995 for all analyses. The limit of detection (LOD) was less or equal to 10 μg/kg for approximate 97.69% pesticides. The limit of quantification (LOQ) was equal to 10 μg/kg for approximate 85.38% pesticides. The attained recoveries were typically between 70% and 120% with relative standard deviation (RSD) values that are typically lower than 20% for about 80%-91.54% pesticides at four spiking levels of 10, 20, 50 and 100 μg/kg. The results are in good agreement and indicate that the proposed method provide to determine the trace level amounts of pesticides residues in Chrysanthemum samples. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。