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題名 | Effects of Ultraviolet B on Cell Motility and Expression of Matrix Metalloproteinase in Liver Cancer Cells=中波紫外線對於肝癌細胞移動性及基質金屬蛋白酶表現之影響 |
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作者 | 林大興; 林麗玲; 李意旻; 黃海義; 吳泰賢; 蔣宜蒼; 林松水; Lin, Tai-shing; Lin, Li-ling; Lee, Yi-min; Hung, Hai-i; Wu, Tai-hsien; Chiang, I-tsang; Lin, Song-shei; |
期刊 | 中華放射線技術學雜誌 |
出版日期 | 20090600 |
卷期 | 33:1 2009.06[民98.06] |
頁次 | 頁69-74 |
分類號 | 415.536 |
語文 | eng |
關鍵詞 | 移動性; 基質金屬蛋白分解酶; 紫外線; 西方墨點法; Cell migration; Matrix metalloproteinase; Ultraviolet; Western blotting; |
中文摘要 | 本研究目的在於探討紫外線處理後對於肝癌細胞(J5 cell)移動性與相關蛋白質包括基質金屬蛋白分解酶(matrix metalloproteinase)、細胞核蛋白B(NF-KB)及Bcl-2等表現之影響。肝癌細胞培養在100平方毫米含新鮮培養液(Ham's F12K medium)的培養瓶中,18小時後移除培養液,且將上蓋移除,利用中波紫外線交聯儀以波長爲312奈米、劑量分別以50、100、150毫焦耳/平方毫米的能量輸出調控所需之劑量暴露。肝癌細胞培養24小時或經輻射暴露後6小時利用西方墨點法、電泳等方法分析MMP,Bcl-2及NF-KB等訊息蛋白之差異性表現。紫外線單獨處理組(150 mJ/平方公分)對照控制組只造成輕微降低其存活率,顯示低劑量紫外線對於肝癌細胞敏感度不佳。三組劑量所造成存活率且都維持在75%以上。紫外線單獨處理組明顯大大刺激細胞移動性,且具時間依賴性及統計上的顯著性差異。顯示紫外線處理下可促進細胞移動性,其Bcl-2和基質金屬蛋白酶(Matrixmetalloproteinase-2, MMP-2)的表現明顯增加,而MMP-9顯示並沒有明顯的增加,顯現低劑量紫外線(UVB)具有增加MMP-2與細胞移動之相對性表現,另外從細胞核蛋白NF kappa B和bcl-2也有劑量依賴性的趨勢,同時呈現訊息蛋白的表現而增加,但並不引導大量增加肝癌細胞凋亡與死亡率,顯示MMP-2的增加可能藉由NF kappa B的表現來媒介細胞移動的機制。低劑量紫外線B(150 mJ/平方公分)的處理明顯增加Bcl蛋白的表現,也顯示低劑量紫外線對肝癌細胞並不敏感。 |
英文摘要 | This study aimed to investigate the cell migration and related protein expression including matrix metalloproteinase (MMP-2 & MMP-9), nuclear fator kappa B (NF-KB), and Bcl-2 protein after different doses of ultraviolet exposure in human liver cancer cell (J5 cells). J5 cells (5×10^5) were plated on 100 mm^2 culture dishes, and approximately 18 h later Ham's F12K medium was removed, and with lids off, cells were exposed to 50, 100, 150 mJ/cm^2 UV light (312 nm) by using a UV gene linker apparatus equipped with an energy output control. The total proteins were collected at 6 h after UV exposure or cultured for 24 hours. MMP, Bcl-2 and NF-KB were assayed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Viability of UV treatments was decreased slightly comparing to the control group. Survival rates of the three doses were over 75%. UV treatment of 150 mJ/cm^2 greatly enhanced cell migration with significant difference of statistics and induced over-expression of bcl-2 and MMP-2. However, MMP-9 was not increased after UV exposure. It appeared that low dose UV to increase the relative effects of MMP-2 expression and cell migration. UV exposure increased the expression of NF-KB and Bcl-2 on dose dependent in liver cancer cells. It demonstrated that UVB increased higher expression levels of NF-KB and Bcl-2 than the control group, which promoted the cell migration in liver cancer cells. MMP-2 level increased by the enhanced expression of NF kappa B may be involved with the migration mechanism. Low dose treatment of 150 mJ/cm^2 UVB increased the Bcl-2 expression apparently and showed that low dose UVB irradiation was not sensitive in liver cancer J5 cell. |
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