頁籤選單縮合
題名 | 廣藿香抗氧化成分之分離純化及結構鑑定=Separation, Isolation and Identification of Antioxidative Components from Pogostemon chablin |
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作者 | 盧錫祺; 喬長誠; 柯宏達; 陳昱翔; 熊皓儀; 蘇正德; Lu, Hsi-chih; Chyan, Charng-cherng; Ke, Hung-ta; Chen, Yu-hsiang; Hsiung, Hao-yi; Su, Jeng-de; |
期刊 | 臺灣農業化學與食品科學 |
出版日期 | 20130600 |
卷期 | 51:3 2013.06[民102.06] |
頁次 | 頁120-126 |
分類號 | 414.33 |
語文 | chi |
關鍵詞 | 廣藿香; 抗氧化成分; 多酚配苷; Pogostemon chablin; Antioxidative component; Polyphenol glycoside; |
中文摘要 | 本研究探討廣藿香(Pogostemon chablin)之抗氧化成分,廣藿香粉末依序以正己烷、乙酸乙酯及甲醇萃取、過濾、濃縮後檢測其抗氧化性,三種溶劑萃取物之抗氧化性依序為BHA>甲醇萃取物>α-生育醇>乙酸乙酯萃取物>正己烷萃取物>控制組。選擇具抗氧化較佳之廣藿香甲醇萃取物,經XAD-7液相層析區分出顯現強抗氧化性的沖提部分,利用高效液相層析法分析,選擇具抗氧化性,且所含成分較不複雜之區分,重複以高效液相層析分離純化,藉各種光譜分析法鑑定,得到三種純化物質,分別為adenosine、4-hydroxyethylcatachol-1-O-(2'-α-L-rhamnopyranosyl)-β-D-glucopyranoside及ο-coumaroyl-β-D-glucopyranoside。 |
英文摘要 | The purpose of this study was to investigate the antioxidative components from Pogostemon chablin. The ground powder of Pogostemon chablin was sequentially extracted with n-hexane, ethyl acetate and methanol. The antioxidative activity of the extract was measured by the ferric thiocyanate method. The order of antioxidative activity was: BHA > methanol extract > α-tocopherol > ethyl acetate extract > n-hexane > control. Due to the strong antioxidative activity of methanol extract, it was fractionated and isolated by various chromatographic method to obtain three pure components identified as adenosine, 4-hydroxyethyl-catachol-1-O-(2'-α-L-rhamnopyranosyl)-β-D-glucopyranoside, ο-coumaroyl-β-D-glucopyranoside. |
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