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| 題 名 | 桑黃(Phellinus linteus)於攪拌式生物反應器中菌絲體生質量及生理活性物質之生產=Production of Mycelial Biomass and Bioactive Compounds by Phellinus linteus in a Stirred-Tank Bioreactor |
|---|---|
| 作 者 | 吳秋曄; 廖姿敏; 梁志欽; 陳淑茹; 宋祖瑩; 梁志弘; 曾慶瀛; | 書刊名 | 臺灣農業化學與食品科學 |
| 卷 期 | 49:6 2011.12[民100.12] |
| 頁 次 | 頁329-337 |
| 分類號 | 379.1 |
| 關鍵詞 | 桑黃; 攪拌式生物反應器; 菌絲體生質量; 生理活性物質; Phellinus linteus; Stirred-tank bioreactor; Mycelial biomass; Bioactive compounds; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 本研究探討桑黃(Phellinus linteus)於5-L攪拌式生物反應器生成菌絲體生質量及生理活性物質(β-1,3-glucan、總酚)之培養條件。試驗結果顯示,P.linteus生成菌絲體的較適接種量、通氣量與攪拌速度分別為5%、1.5vvm與125rpm。P.linteus於上述較適條件培養20天,其最大菌絲生成量、菌絲體生成速率與菌絲體產率分別為8.86g dry mycelia biomass/L、0.44g dry mycelia biomass/L/day與0.44g/g glucose,與培養於基礎發酵條件(接種量為10%、通氣量1.0vvm與攪拌速度100 rpm)比較,培養於較適條件之最大菌絲體生成量為基礎發酵條件的1.93倍,菌絲體生成速率與菌絲體產率則為1.91倍。在20天發酵期間發酵液之總酚含量與總抗氧化能力隨P.linteus菌絲體生成量增加而增加,但是β-1,3-glucan濃度隨培養時間增長而減少。 |
| 英文摘要 | The influences of cultivation conditions on mycelial biomass and bioactive compounds produced by a medicinal mushroom Phellinlls Iinleus in a 5-L stirred-tank bioreactor were studied. The results showed that the optimal inoculum ratio, aeration rate and agitation rate for the production of mycelia biomass were 5%, 1.5 vvm and 125rpm, respectively. Then, the mushroom was grown under the optimal cultivation conditions (inoculum ratio 5%, aeration rate 1.5vvm and agitation rate 125 rpm) in a 5-L stirred-tank bioreactor after 20 days of incubation time, and the maximal production of mycelial biomass, mycelial biomass formation rate and mycelial biomass yield were 8.86g/L, 0.44g/L/day and 0.44g/g glucose, respectively. These results were 1.93-, 1.91- and 1.91-fold of the maximal production of mycelial biomass, mycelial biomass formation rate and mycelial biomass yield of P Iinleus under the basal cultivation conditions (inoculums ratìo 10%, aeration rate 1.0vvm and agitation rate 100 rpm). During 20 days of incubation time, the total phenolic content and values of Trolox equivalent antioxidative capacity for P. Iinlcns culture broth were increased with production of mycelia biomass. However, the content of ß-1,3-glucan was decreased during incubation time. |
本系統中英文摘要資訊取自各篇刊載內容。