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題 名 | 篩選適合落花生毛狀根生長之簡化培養基=Selection of Simplified Medium Suitable for Hairy Root Growth of Peanut |
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作 者 | 蔡杏枚; 廖宇賡; | 書刊名 | 作物、環境與生物資訊 |
卷 期 | 8:4 2011.12[民100.12] |
頁 次 | 頁242-253 |
分類號 | 434.257 |
關鍵詞 | 落花生; 毛狀根; 花寶1號; 馬鈴薯抽出物; 簡化培養基; Arachis hypogaea; Hairy root; Hyponex 1; Potato extract; Simplified medium; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究試圖從開發簡化培養基(simplified medium)著手,降低落花生(Arachis hypogaea L.)毛狀根(hairy root)大量培養時的成本支出。先以農桿根群菌(Agrobacterium rhizogenes)BCRC 15010(ATCC 15834)感染落花生臺南選9號無菌苗葉片培殖體,以誘導所得的毛狀根作為試驗材料,在數種農、園藝用速溶性化學肥料調製的培養基中進行繼代培養,比較以這些化學肥料配製的不同濃度培養基對毛狀根生長產生的效應,選擇適當配方,再配合加入有機添加物,觀察毛狀根生長狀態以決定最佳組合。研究結果顯示,最佳簡化培養基是以花寶1號(H-1)肥料取代無機鹽類,並加入馬鈴薯抽出物(PE)作為有機添加物配製而成。落花生毛狀根在本配方及MS 基礎培養基中生長(增重)並無顯著差異(p > 0.05),然簡化培養基中所添加項目之市場價購支出僅為MS培養基之8%,可有效降低消耗藥品的採購支出,有利於商業化生產。 |
英文摘要 | In the present study, a cost effective simplified medium for culturing hairy roots of peanut (Arachis hypogaea L.) was developed. The Agrobacterium rhizogenes BCRC 15010 (ATCC 15834) was first applied to infect leaf explants of in vitro grown peanut plants (Tainan Selected 9; TNS 9), from which the hairy roots were induced as experimental materials. Hairy root cultures were then incubated on media made of instant soluble inorganic fertilizers with designated concentrations. The Concentrations of better combinations were chosen for further investigation of the promotion effect of organic supplements. The results revealed that the best medium composition was that adding Hyponex No. 1 fertilizer (H-1) as replacement of inorganic salts and potato extract (PE) as organic supplement. The growth of hairy roots under this simplified medium exhibited similar fresh weight increase (p > 0.05) as those grown under MS medium. The price for purchasing the additives for preparing the simplified medium is only 8% of the expenditure for those needed in preparation of MS medium. Consequently this medium can be considered a low cost formula for commercial use. |
本系統中英文摘要資訊取自各篇刊載內容。