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題 名 | Using Orthogonal Arrays to Obtain Efficient and Reproducible Extraction Conditions of Geniposide and Genipin in Gardenia Fruit with Liquid Chromatography-Mass Spectrometry Determinations=使用正交陣列獲致從梔子果實中萃取去羥梔子苷與梔子平的高效率與高重複性實驗條件並以層析質譜術定量 |
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作 者 | 王少君; 李書吉; 洪嘉鴻; 廖慧如; 黃志民; 蔡東湖; | 書刊名 | Journal of Food and Drug Analysis |
卷 期 | 19:4 2011.12[民100.12] |
頁 次 | 頁486-494+541 |
分類號 | 418.52 |
關鍵詞 | 正交陣列; 去羥梔子苷; 梔子平; 層析質譜; 梔子; Orthogonal array; Geniposide; Genipin; LC-MS; Gardenia fruit; |
語 文 | 英文(English) |
英文摘要 | The conditions to efficiently and reproducibly extract geniposide and genipin in Gardenia fruit powders were established using Taguchi experimental design approaches to improve extraction recovery. Geniposide, the major iridoid glycoside component of Gardenia fruits, which has been recognized to have choleretic effects, is transformed to Genipin in animals. In this study, the extraction conditions of geniposide and genipin such as solvent types, solvent acidities and boiling times were investigated under the guidance of experimental designs to obtain the highest content in extracts determined with liquid chromatography-mass spectrometry (LC-MS) methods. Herbal powders ground from Gardenia fruits, Gardenia jasminoides Ellis and Gardenia jasminoides Ellis var. grandiflora Nakai, were used. This paper has successfully demonstrated the use of Taguchi orthogonal arrays to obtain proper extraction conditions of geniposide and genipin in the above fruits. These design methods are capable of obtaining the optimum extraction conditions in only nine trials in which three factors, namely solvent type, boiling time and solvent acidity are investigated. The results show that solvent acidity is the influencing factor in achieving adequate contents and the other factors only have minor influences. The highest content obtained is similar to the reported data. In addition, using Taguchi orthogonal arrays within four experimental trials has identified that the extraction of ground fine powders results in more reproducible recovery than that of coarse ones. |
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