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題 名 | In Vitro Evaluation of Antioxidant Activities and Inhibition of ACE Activity by Collagenase-Treated Hydrolysate Derived from Bullfrog Skin=以體外試驗評估牛蛙(Rana catesbeiana)皮酵素水解物之抗氧化活性及膠原蛋白酶水解物對血管收縮素轉化酶(ACE)的抑制活性 |
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作 者 | 黃雅玲; 李淑惠; 莊子儀; 周照仁; | 書刊名 | Journal of Food and Drug Analysis |
卷 期 | 19:3 2011.09[民100.09] |
頁 次 | 頁301-308+378 |
分類號 | 412.37 |
關鍵詞 | 牛蛙; 明膠水解液; 酵素水解; 抗氧化活性; 血管收縮素轉化酶抑制活性; Bullfrog; Gelatin hydrolysate; Enzymatic hydrolysis; Antioxidant activity; ACE inhibitory activity; |
語 文 | 英文(English) |
英文摘要 | ABSTRACT Enzymatic hydrolysates were obtained by hydrolysis of retorted skin gelatin hydrolysates (RSGH) from bullfrog (Rana catesbeiana) using three enzymes including collagenase, bromelain, and pancreatin for various times (0, 15, 30 and 60 min). As the hydrolysis time increased, degrees of hydrolysis (DH) of all three enzymatic hydrolysates increased. The molecular weight distribution of collagenase-hydrolyzed RSGH (RSGH-Cg) fraction was in the range of 189 - 686 Da (40.1 - 50.1%) and 686 - 6,511 Da (36.7 - 48.7%) while those of the other enzymatic-hydrolyzed RSGH fractions were mostly in the 686 - 6,511 Da (58.2 - 60.8%) range. All enzymatic hydrolysates had more potent antioxidant activity including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferrous ion chelating activity, and reducing power than the original RSGH. RSGH-Cg showed the highest activity. The lower IC50 value of angiotensin I-converting enzyme (ACE) inhibitory activity of RSGH-Cg correlated with the degree of hydrolysis. This study suggested that collagenase treated bullfrog RSGH is a promising functional ingredient. |
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