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題名 | Increased Cellulose Production by Heterologous Expression of Cellulose Synthase Genes in a Filamentous Heterocystous Cyanobacterium with a Modification in Photosynthesis Performance and Growth Ability=絲狀藍綠菌表現異源纖維素合成酶基因促進纖維素合成、光合作用與生長 |
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作者姓名(中文) | 蘇翔筵; 李澤民; 黃郁儒; 周聖炘; 王嘉寶; 林立夫; 周德珍; | 書刊名 | Botanical Studies |
卷期 | 52:3 2011.07[民100.07] |
頁次 | 頁265-275 |
分類號 | 379.32 |
關鍵詞 | 纖維素; 纖維素合成酶; 藍綠菌; 光合作用; Cellulose; Cellulose synthase; Cyanobacteria; Photosynthesis; |
語文 | 英文(English) |
中文摘要 | 微藻的纖維素與醣可用來生產生質酒精。而提高微藻的纖維素與醣的合成能力是以微藻大量生產生質酒精的關鍵。為了增加纖維素產量,我們將Acetobacter xylinum纖維素合成酶基因acsAB以接合生殖方式轉入藍綠菌Anabaena sp. strain PCC7120細胞中。PCR分析結果確認轉殖株具有acsAB基因,而RT- PCR分析亦檢測出轉殖株中acsAB基因之表現。轉殖株培養液分別以Calcofluor white染色,及聯結FITC的纖維素水解酶CBHI-FITC為探針檢測纖微素,皆發現acsAB轉殖株細胞外物質染色後出現螢光,顯示PCC7120 acsAB基因轉殖株分泌胞外纖維素。而與wild type相比,acsAB基因轉殖株經纖維素水解後的葡萄糖含量亦顯著的增加。轉殖株之光合作用效率分析結果顯示轉殖株的光合作用效率及生長速率提高。綜上所述,Anabaena sp. strain PCC7120表達acsAB不僅提高了纖維素的合成,對其光合作用及生長亦無負面影響。 |
英文摘要 | Cellulose and sugar from microalgae can be utilized for the production of biofuel ethanol. Increasing cellulose and sugar synthesis capacity is key for high yield production of this biofuel. To enhance cellulose production, we transferred acsAB, responsible for cellulose synthesis in bacterium Acetobacter xylinum, into the cyanobacterium, Anabaena sp. strain PCC7120, by conjugation. PCR confirmed the presence of acsAB in Anabaena sp. strain PCC7120 exconjugates. RT-PCR demonstrated the up-regulation of acsAB expression. Production of extracellular cellulose secreted from Anabaena sp. strain PCC7120 carrying acsAB was revealed using Calcofluor white staining and cellobiohydrolase I (CBHI)-FITC labeling. Further evidence obtained from the digestion of cellulose to glucose demonstrated that the amount of glucose released from cellulose was significantly increased in Anabaena sp. PCC7120 cells carrying acsAB as compared to the wild type. The photosynthetic efficiency and growth rate were increased in the transgenic strains. Cellulose synthesis is thus enhanced in Anabaena sp. strain PCC7120 expressing acsAB and does not negatively impact photosynthesis and growth. |
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