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題 名 | Isolation and Characterization of a Polycyclic Aromatic Hydrocarbons-degrading Enzyme from Xylaria regalis=炭角菌分解多環芳香族化合物酵素之分離及其生化特性之探討 |
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作 者 | 張勝祺; 陳明凱; 魏玎玲; 鄭閔魁; | 書刊名 | 中華真菌學會會刊 |
卷 期 | 22:1/2 2007.07[民96.07] |
頁 次 | 頁25-33 |
分類號 | 379.1 |
關鍵詞 | 炭角菌; 多環芳香族化合物; 多環芳香族化合物分解酵素; Polycyclic aromatic hydrocarbons; PAHs; PAHs-degrading enzyme; Xylaria regalis; |
語 文 | 英文(English) |
中文摘要 | 從21株真菌篩選分解多環芳香族化合物Pyr及BaP之能力,發現炭角菌(Xylaria regalis)分解能力最強。進一步從其菌絲分離及純化此酵素,經由離子交換樹脂管柱、疏水性管柱及分子篩膠體管柱之純化,得到最終產物活性提高70.5倍,回收率45%,專一活性4690U/mg;經由SDS-膠體電泳分析得知此酵素分子量為29KDa。此酵素最適反應Ph值8.5,最適反應溫度30℃,且在高溫相當穩定,在100℃反應8小時仍保持60%之活性。 |
英文摘要 | Twenty-one fungal species were screened for capability of removing pyrene and benzo (a) pyrene in culture. Xylaria regalis was found to show the highest activity. An PAHs-degrading enzyme was purified to homogeneity from the culture mycelium of X. regalis. The crude enzyme was subjected to a series of purification procedures, including QAE Sephadex A-25 anion exchange column, hydrophobic interaction phenyl 650M column and Fractogel HW-55 column chromatography, respectively. The enzyme was purified 70.5 fold, giving a 45% yield. The specific activity was 4690U/mg. The molecular mass of the purified PAHsdegrading enzyme estimated by SDS-PAGE was approximately 29kDa. The optimal pH of the purified enzyme was 8.5 and the enzyme was stable between pH 8.0-9.0. The optimal temperature of the enzyme was 30℃ and it's activity was still stable at higher temperatures. The enzyme was found stable within 2h at 100℃ and retained 60% of activity after 8 h incubation. |
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