查詢結果分析
來源資料
相關文獻
- Purification and Characterization of Xylanase from Xylaria Regalis
- Xylariaceous Mycobiota in Taiwan: An Overview
- 福山植物園及其真菌資源
- Xylanase and β-xylosidase Production by Xylaria spp.
- Clarification and Typification for Xylaria aenea
- Isolation and Characterization of a Polycyclic Aromatic Hydrocarbons-degrading Enzyme from Xylaria regalis
- Purification and Characterization of β-xylosidase from an Isolated Xylaria regalis 76072314
- Xylaria terricola sp. nov., a Terrestrial Anamorphic Xylaria Species found in Taiwan
- Xylaria botuliformis, a Small-ascospored Species with a Reticulately Cracked Stromatal Surface
- 臺灣香菇段木上炭角菌之鑑定與藥劑感受性試驗
頁籤選單縮合
題名 | Purification and Characterization of Xylanase from Xylaria Regalis=炭角菌木聚糖酵素之純化及其生化特性之探討 |
---|---|
作者 | 魏玎玲; 周慧琴; 鄭閔魁; 張勝祺; | 書刊名 | 中華真菌學會會刊 |
卷期 | 20:1/2 民94.06 |
頁次 | 頁53-59 |
分類號 | 379.1 |
關鍵詞 | 木聚糖酵素; 炭角菌; Xylanase; Xylaria regalis; |
語文 | 英文(English) |
中文摘要 | 炭角菌(Xylaria regalis)產生之木聚糖酵素經由離子交換樹脂管柱、疏水性管柱及分子篩膠體管柱之純化,得到最終產物活性提高10.98倍,回收率為34.6%。經由SDS-膠體電泳分析得知此酵素分子量為30.8kDa;此外,本酵素最適反應溫度50℃,最適反應pH值5.5。金屬離子Cu(上標 2+)、Hg(上標 2+)及化合物EDTA或β-mercaptoethanol會抑制此酵素之活性。 |
英文摘要 | An extracellular xylanase was purified to homogeneity from the culture filtrate of "Xylaria regalis". The xylanase was purified 10.98 fold, giving a 34.6% yield. The crude enzyme was subjected to a series of the purification procedures, including QAE Sephadex A-25 anion exchange column, hydrophobic interaction phenyl 650M column and Sephadex G-75 gel filtration column chromatography, respectively. The molecular mass of the purified xylanase estimated by SDS-PAGE was approximately 30.8 kDa. The optimal temperature of the enzyme activity was 50℃. The optimal pH of xylanase activity was 5.5 and the enzyme appeared to be stable over the pH range from 5-9. The enzyme activity was inhibited by Cu(superscript 2+), Hg(superscript 2+), EDTA, and β-mercaptoethanol. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。