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題 名 | 應用寡核酸探針偵測生物分解PAH/界面活性劑之微生物族群結構=Bacterial Community Dynamics of PAH/Surfactant Biodegradation Detected by Oligonucleotide Probes |
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作 者 | 張怡塘; 李俊福; 康閎洋; 王灝頤; 葉榮美; 詹惠蘋; 乜怡萱; 顏秀芳; 鄭雅文; 施孟岑; 陳清; | 書刊名 | 東南學報 |
卷 期 | 27 2004.12[民93.12] |
頁 次 | 頁261-270 |
分類號 | 445.259 |
關鍵詞 | 界面活性劑淋洗技術; 多環芳香族化合物; 螢光原位雜交技術; Polycyclic aromatic hydrocarbons; Surfactant flushing technology; Fluorescence in-situ hybridization; |
語 文 | 中文(Chinese) |
中文摘要 | 多環芳香族化合物(PAH)為土壤與地下水中經常發現之有機污染物,環境工程師經常結合生物復育與界面活性劑淋洗技術去除PAH,但其微生物可能受添加之界面活性劑影響,促進或抑制PAH之生物分解,進而造成微生物族群結構的改變。本研究以螢光原位雜交技術(FISH)偵測含界面活性劑下生物分解PAH之微生物族群結構。研究結果顯示生物分解含Triton X-100或Brij 35對生物分解菲(phenanthrene)分別為促進與抑制,並與環境中所含界面活性劑的濃度與微胞(micelle)無關。生物分解菲至之過程(濃度C/Co=0.05)之族群分佈包括62.90%細菌域(Bacteria)與34.59%古細菌域(Archaea);其中Bacteria包括環境中常見的5類微生物,其所佔比例分別為:14.86% α-Proteobacteria、10.84% β-Proteobacteria、12.17%γ-Proteobacteria、13.60% Gram positive bacteria with High G+C DNA content(HGC)與11.14% Gram positive bacteria with Low G+C DNA content(LGC)。添加界面活性劑並不會造成這些族群消失,但其比例會發生增減。若以特定種探針(species-specific probe)分析其族群,可發現:(1)α-Proteobacteria之Brevundimonas(Pseudomonas)diminuta, Caulobacter sp., Mycoplana bullata;(2)β-Proteobacteria之Burkholderia sp與Acidovorax sp.;(3)γ-Proteobacteria之Pseudoomonas aeruginosa等族群扮演著重要角色,添加界面活性劑下則以假單胞菌屬變動較為顯著,本研究建議選擇包括Pdi與PseaerA寡核酸探針可選擇用來偵測生物復育PAH/界面活性劑過程之異動。 |
英文摘要 | Polycyclic aromatic hydrocarbons (PAH) are one of common organic pollutants found in soil and groundwater. Environmental engineers is able to remove PAH effectively by integrating bioremediation with surfactant flushing technology. The process would increase or decrease PAH biodegradation according to previous researches. The microorganism community might be changed when surfactant is added. This study is seeking for the bacterial community dynamics by fluorescence in-situ hybridization if PAH biodegradation containing surfactant with monomer or micelle. The experimental results indicated that the rate of phenanthrene biodegrading with Triton X-100 was increased but that with Brij 35 was decreased. The structure of surfactant in aqueous, monomer or micelle seemingly was no related to phenanthrene biodegradation. Bacterial community composed of 62.90% Bacteria and 34.59% Archaea since the ratio of remaining phenanthrene concentraion to initial concentration (C/Co) was 0.05. Five phylum and subclass probes commonly found in environment were detected during biodegradation, including 14.86% α-Proteobacteria, 10.84% β-Proteobacteria, 12.17% γ-Proteobacteria, 13.60% Gram positive bacteria with High G+C DNA content (HGC) and 11.14% Gram positive bacteria with Low G+C DNA content (LGC). The percentage of bacterial community was increasing or decreasing if surfactant additive was applied, instead of all disappearing. Futher to detecting species-specific probe, this study found following important species, including (1) Brevundimonas (Pseudomonas) diminuta, Caulobacter sp., Mycoplana bullata, belonged to α-Proteobacteria; (2) Burkholderia sp. and Acidovorax sp., belonged to β-Proteobacteria; (3) Pseudomonas aeruginosa, belonged to γ-Proteobacteria. Porbe Pdi and PseaerA were recommended to monitor the bacterial community during PAH/Surfactant biodegradation. |
本系統中英文摘要資訊取自各篇刊載內容。