查詢結果分析
相關文獻
- Effect of Flavonoids on Heavy Metal Tolerance in Arabidopsis Thaliana Seedlings
- 從事劇烈運動需要補充硫醇性的抗氧化劑:穀胱甘冴
- 抗氧化劑對肉品氧化穩定性之影響
- β-胡蘿蔔素對消除運動氧化壓力的探討
- 棉染色織物因包裝膠帶產生變褪色之研究
- 蜂膠性質評量:抗氧化作用
- 生物固氮研究之進展:根瘤菌與豆科植物共生過程中複雜的交互作用
- 抗氧化劑與心血管疾病的關係
- Characterization of the Thylakoid Membrane in a Chlorophyll-Deficient ch5 Mutant of Arabidopsis Thaliana
- The Processing of Metabolized Low Density Lipoproteins in Foam Cells Generates Superoxide Anions
頁籤選單縮合
題名 | Effect of Flavonoids on Heavy Metal Tolerance in Arabidopsis Thaliana Seedlings=類黃素對阿拉伯芥幼苗之重金屬耐受性之效果 |
---|---|
作者 | Keilig, Katharina; Ludwig-Müller, Jutta; | 書刊名 | Botanical Studies |
卷期 | 50:3 2009.07[民98.07] |
頁次 | 頁311-318 |
分類號 | 435.242 |
關鍵詞 | 抗氧化劑; 阿拉伯芥; 重金屬耐受性; 類黃素; Phytochelatin 合成酶; 透明種臍之突變種; Antioxidant; Arabidopsis thaliana; Heavy metal tolerance; Flavonoids; Phytochelatin synthase; Transparent testa mutants; |
語文 | 英文(English) |
中文摘要 | 本文使用阿拉伯芥為材料來檢驗類黃素對它的重金屬耐受性之可能影響。野生型WT 及突變種 (tt, 類黃素之生合成受損者) 分別栽培於含不同重金屬之培養基並且評估兩種生長參數。當Cd 存在時,tt 之根長及幼苗重都比WT 減少; 當Zn 存在時只有根長減少。做為重金屬耐受性之標誌,我們以 RTPCR 定量兩種 phytochelatin synthase 基因 (AtPCS1, AtPCS2) 之誘導。在較高濃度之Cd 存在下,AtPCS1 之轉錄產物在WT 高於tt5 ; 相反地,AtPCS2 之轉錄產物並未在WT 產生,但卻在tt5 產生。接著我們檢查突變種之生長受抑制之表現型可否以添加類黃素之方式來補救。為此,幼苗分別以 flavanone naringenin 及 flavonol quercetin 處理。對WT 而言,quercetin 及 naringenin 在Zn 濃度高達 500 µM 時仍可促進根之生長及幼苗之鮮重,然而對比而言,卻無效果。可是幼苗重有所改善,尤其是tt5 。當Cd 存在時,只有tt5 之根長缺陷可被改善,而幼苗重在所有品系之植物都有改善,只是程度不同而已。上述結果以 flavonoid 在重金屬耐受性方面之潛在功能予以討論。 |
英文摘要 | Arabidopsis thaliana was used to investigate possible effects of flavonoids on heavy metal tolerance. Arabidopsis wild type (WT) and mutant lines with a defect in flavonoid biosynthesis (tt) were grown on media containing different heavy metals, and two growth parameters were evaluated. It was shown that root length and seedling weight were reduced in mutants more than in the wild type when grown on cadmium (Cd) while on zinc (Zn) only root length was affected. As marker for heavy metal tolerance, the induction of two phytochelatin synthase genes (AtPCS1, AtPCS2) in WT and the tt5 mutant after Cd treatment was monitored by quantitative RT-PCR. The AtPCS1 transcript was induced in WT at higher Cd levels than in the mutant. In contrast, the AtPCS2 transcript was not induced under these experimental conditions in the WT, but it was induced in tt5. We then investigated whether the growth inhibition phenotype of the mutants could be rescued by addition of flavonoids. For this, the seedlings were treated with the flavanone naringenin and the flavonol quercetin, and the same growth parameters were determined. Quercetin and naringenin induced root growth and seedling fresh weight of WT on concentrations of Zn up to 500 µM, but this was not the case for root length of tt mutants. However, seedling weight could be stimulated by flavonoids, especially in the tt5 mutant line. When the plants were grown on Cd, only the root length defect of tt5 plants could be rescued by flavonoids. The seedling weight increased on Cd in all lines, although to a different extent. These results are discussed with respect to the potential role of flavonoids in heavy metal tolerance. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。