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題 名 | Arabidopsis Floral Regulators FVE and AGL24 are Phloem-mobile RNAs=阿拉伯芥開花調控基因 FVE 與 AGL24 為篩管中之移動性 RNA |
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作 者 | 楊鴻偉; 余天心; | 書刊名 | Botanical Studies |
卷 期 | 51:1 2010.01[民99.01] |
頁 次 | 頁17-26 |
分類號 | 373.1 |
關鍵詞 | 阿拉伯芥; 青花菜; 開花; 嫁接; 篩管液; RNA 長距離移動; Arabidopsis; Brassica oleracea; Flowering; Grafting; Phloem sap; RNA long-distance movement; |
語 文 | 英文(English) |
中文摘要 | 植物運用維管束系統來面對環境中多樣的刺激,以調節自身的生長發育。近來的研究證實,植物在葉部整合光週期的變化並利用維管束系統長距離輸送 FLOWERING LOCUS T (FT) 至頂芽,促進植物的開花。因此,FT 被認為是追尋已久的開花素(Florigen)。然而,影響植物開花的因素除了光週期外,證據顯示尚有其他基因在植物開花的路徑中扮演系統性的調控因子。我們分析青花菜收取之篩管液,企圖找出潛在的系統性開花調控因子。利用基因微陣列 (Microarray) 及反轉錄聚合酶連鎖反應 (RT-PCR) 的分析方法,從青花菜篩管液中鑑定出 FVE 與AGAMOUS-LIKE 24 (AGL24) 兩個與開花調控有關的 RNA 。酵素學上的分析證明 FVE 與 AGL24 的 RNA 帶有 5'-cap 結構及 polyadenylation tail ,為典型之訊息 RNA (mRNA) 。文獻資料顯示,大量表現 FVE 與 AGL24 可促進植物提早開花。我們以野生型阿拉伯芥為接穗嫁接至 FVE 或 AGL24 轉殖株系之砧木上。利用 RT-PCR 的方式,可由野生型接穗偵測出 FVE 或 AGL24 轉殖基因之 RNA 。顯示FVE 與AGL24 RNA 可經由維管束系統進行長距離運送。我們的實驗結果顯示,除了FT ,尚有多個基因對於植物開花具有系統性的調控。 |
英文摘要 | Plants take advantage of the vascular system to operate environmental stimulates for finetuning their developmental programs. Recent evidence shows that the FLOWERING LOCUS T (FT) protein is the long-sought-after florigen that integrates the photoperiod variation perceived in the leaves. However, evidence also supports that other yet-to-be identified systemic regulators participate in floral induction. To this end, we investigated phloem exudates from excised broccoli (Brassica oleracea) inflorescences. Microarray and RT-PCR analyses revealed that at least two RNAs of floral regulators, FVE and AGAMOUS-LIKE 24 (AGL24), are present in the phloem sap. Enzymatic analysis demonstrated that the phloem-sap RNAs contain a 5´ cap and a polyadenlylation tail, which suggests that phloem sap contains typical mRNAs. Arabidopsis grafting experiments were used to test whether these RNAs move long distance along the phloem translocation stream. Consistent with previous reports, Arabidopsis transformants expressing FVE and AGL24 displayed an early flowering phenotype. When wild-type scions were grafted onto P35S -FVE or P35S -AGL24 transformant stocks, the RNAs of transgenic FVE and AGL24 were detected from the wild-type scions. Thus, both FVE and AGL24 RNAs can move long distance across the graft union. Our data support the notion that multiple systemic floral regulators may participate in floral regulation. |
本系統中英文摘要資訊取自各篇刊載內容。