查詢結果分析
來源資料
相關文獻
- 果莢成熟度及氟啶草酮處理對黃萼捲瓣蘭無菌播種發芽之影響
- 培養基組成及培養密度對黃根節蘭種子發芽與小苗生育之影響
- Embryonic Cell Lines Derived from Morula to Late Hatched Blastocyst Stages in Pigs
- 落花生幼齡子房柄的體外培養(1)--Kinetin及抗壞血酸對莢果形成及胚發育之效應
- 臺灣雲杉胚原性癒合組織體胚發育之變異及其與生長素相關性之探討
- 哺乳動物胚發育期對熱緊迫之反應
- 經體外成熟和體外受精之牛卵母細胞與卵丘細胞共培養之發育率
- 有機添加物Glutamine對花生子房柄培養後結莢及胚發育之影響
- 大豆屬種間雜交胚發育的研究
- 文心蘭蒴果和胚的成熟度對種子發芽之影響
頁籤選單縮合
| 題 名 | 果莢成熟度及氟啶草酮處理對黃萼捲瓣蘭無菌播種發芽之影響=Effects of Capsule Maturity and Fluridone Treatment on in Vitro Seed Germination in Bulbophyllum retusiusculum |
|---|---|
| 作 者 | 馬若婷; 張耀乾; | 書刊名 | 臺灣園藝 |
| 卷 期 | 56:1 2010.03[民99.03] |
| 頁 次 | 頁35-44 |
| 分類號 | 435.431 |
| 關鍵詞 | 豆蘭; 離層酸抑制劑; 非共生發芽; 胚發育; Bulbophyllum; Abscisic acid inhibitor; Asymbiotic germination; Embryo development; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 黃萼捲瓣蘭(Bulbophyllum retusiusculum Reichb. f.)爲臺灣原生豆蘭屬植物,人工繁殖方法之建立將有助於物種之保育,本研究探討果莢成熟度與氟啶草酮(fluridone; 離層酸生合成抑制劑)處理對黃萼捲瓣蘭無菌播種發芽之影響。取自然授粉之果莢進行無菌播種,果莢開裂當天播種者之發芽率最低(27.9%),而果莢開裂前85天播種者之發芽率最高(50.4%)。另取人工授粉後120、150、180及270天之果莢進行無菌播種,結果以人工授粉後270天之果莢發芽率最高(72.6%);其果莢之成熟度相當於開裂前90天。黃萼捲瓣蘭於授粉後120-150天受精,150天左右形成3個細胞的原胚,180天可觀察到發育至早期球形胚階段的原胚,270天胚發育至圓球胚階段。於授粉後130天對黃萼捲瓣蘭果莢處理不同濃度之fluridone不會影響採收果莢之大小及鮮重,但處理50或100 μM fluridone可有效提高種子發芽率;其中以50μM fluridone處理者之發芽率最高,爲33.4%,而未處理者僅15.6%。 |
| 英文摘要 | Bulbophyllum retusiusculum Reichb. f. is an orchid species native to Taiwan. The establishment of mass production method will assist the conservation of this species. The objectives of this study were to determine the effects of capsule maturity and fluridone (an abscisic acid synthetic inhibitor) treatment on in vitro seed germination in B. retusiusculum. Seeds collected and sowed on the day of capsule splitting had the lowest germination percentage (27.9%), while those collected and sowed 85 days before capsule splitting had the highest (50.4%). In another experiment, capsules were harvested 120, 150, 180, or 270 days after artificial pollination (DAP) and the seeds were used for in vitro germination test. Seeds from capsules collected 270 DAP, which was equivalent to 90 days before capsule splitting, had the highest germination percentage (72.6%). In the embryo development of B. retusiusculum, fertilization occurred 120-150 DAP; zygote developed into three-celled proembryo approximately 150 DAP; early globular stage proembryo was discernible 180 DAP; and the embryo developed to the globular stage 270 DAP. Fluridone application at 130 DAP did not affect the size and fresh weight of capsules at harvest; and seeds in capsules treated with 50 or 100 μM fluridone had higher germination rates subsequently. The highest germination percentage was found in seeds from capsules treated with 50 μM fluridone. Seeds so treated had 33.4% germinated while those untreated had only 15.6% germinated. |
本系統中英文摘要資訊取自各篇刊載內容。