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題 名 | 液化澱粉芽孢桿菌BPD1發酵配方測試=Test of Fermentation Formulae Using Bacillus amyloliquefaciens BPD1 |
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作 者 | 林崇寶; 方關珽; 許資敏; 楊雯馨; 胡淼琳; | 書刊名 | 農林學報 |
卷 期 | 63:4 2014.12[民103.12] |
頁 次 | 頁247-255 |
分類號 | 463.8 |
關鍵詞 | 液化澱粉芽孢桿菌; 發酵; 益生菌; Bacillus amyloliquefaciens; Fermentation; Probiotics; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究針對液化澱粉芽孢桿菌 BPD1進行發酵配方試驗,初期以搖瓶進行培養試驗,發現最適配方為糖蜜0.5%、乳糖1%和大豆分離蛋白 2%。以此配方進行培養條件測試,結果顯示接種 24小時內菌數提升 1000倍,24至 48小時菌數提升 10倍菌數無顯著差異。其他培養條件如培養溫度於25℃和 30℃、不同 pH值對菌數則皆無影響。透過 10公升發酵槽的試驗,顯示以糖蜜 0.5%、乳糖1%和大豆分離蛋白2%作為培養基,轉速 200至 300 rpm、溫度 30℃,可得到菌數 4×10 9 CFU/mL以上之發酵液。利用 1噸大型發酵槽以相同的培養基培養,發酵條件為溫度30℃、轉速 160至 200 rpm、通氣量 0.5 vvm,其菌數可達 1.2×10 9CFU/mL以上;修改培養基配方後,利用 1噸大型發酵槽以糖蜜 3%、大豆分離蛋白1%和硫酸亞鐵 0.045%培養基培養,發酵條件為溫度 30℃、轉速 170 rpm、通氣量 0.5 vvm,其菌數可達 1.4×10 9CFU/mL,此配方改善高泡問題,減少消泡劑之使用,降低成本。本試驗說明此一發酵配方可以用來大量生產液化澱粉芽孢桿菌。 |
英文摘要 | This project aimed at using Bacillus amyloliquefaciens (BPD1) to conduct fermentation formula test. At the beginning, we engaged in culture test by using shake flask and found that the best formula was 0.5% molasses, 1% lactose, and 2% isolated soy protein. Then we conducted culture condition test with this formula and found that bacterial count increased 1000 times within 24 hours and then 10 times between 24-48 hours, which showed no significant differences. Other culture conditions such as temperature of 25℃ or 30℃ and pH value made no differences to bacterial count. Through test of 10-liter fermentation tank, BPD1 reached ≥ 4×109 CFU/mL by using 0.5% molasses, 1% lactose, and 2% isolated soy protein as culture medium, with stirring speed from 200 to 300 rpm and temperature of 30℃. As for 1-ton fermntation tank, by using the same culture medium, BPD1 reached ≥ 1.2 × 109 CFU/mL with temperature of 30℃, 160 rpm, and ventilation 0.5 vvm. After adjusting formula, BPD1 reached ≥ 1.4 × 109 CFU/mL by using 1-ton fermntation tank and culture medium of 3% molasses, 1% isolated soy protein, and 0.045% FeSO4, with temperature of 30 ℃, 170 rpm, and ventilation 0.5 vvm. This formula improves foaming problem, cutting down on antifoaming agent and lowering the cost. Overall, this study shows that the fermentation formula is satisfactory for mass production of BPD1. |
本系統中英文摘要資訊取自各篇刊載內容。