頁籤選單縮合
題 名 | Characterization of MRT, a New non-LTR Retrotransposon in Monascus Spp=MRT,紅麴菌中新穎的non-LTR逆轉位子之特徵分析 |
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作 者 | 陳煜沛; 曾慶平; 廖麗玲; 王俊霖; 袁國芳; | 書刊名 | Botanical Studies |
卷 期 | 48:4 2007.10[民96.10] |
頁 次 | 頁377-385 |
分類號 | 369.36 |
關鍵詞 | 紅麴菌; 細菌人工染色體基因庫; 親緣分析; Non-LTR逆轉位子; Bacterial artificial chromosome; Monascus pilosus; Non-LTR retrotransposon; Phylogenetic analysis; |
語 文 | 英文(English) |
中文摘要 | 本研究於紅麴菌中發現新的 non-LTR 逆轉位子,並命名為 MRT。其序列長度約為 5.5 kb,包含有兩個開放讀架 (ORFs)。此兩個開放讀架與 gag 及 pol 基因相似,並且於 pol 相似基因的 3' 端具有很多的腺嘌呤。第一個開放讀架為 517 個氨基酸的蛋白質,包含有多量半胱氨酸的鋅指區域。第二個開放讀架為 1181 個氨基酸的蛋白質,包含有脫嘌呤/脫嘧啶核酸內切酶、反轉錄酶、核糖核酸酶 H 及 CCHC 區域。根據 MRT non-LTR 逆轉位子的氨基酸序列所建立的親緣關係,可將其歸屬於 Tad1 群叢。南方雜交法則進一步發現只有四種紅麴菌 M. pilosus,M. ruber,M. sanguineus 與 M. barkeri 具有 MRT non-LTR逆轉位子。除此之外,利用 β-tubulin 基因所建立的親緣關係則可歸群出 MRT non-LTR 逆轉位子之存在與否。 |
英文摘要 | A new non-LTR retrotransposon, named MRT, was discovered in the filamentous fungus Monascus pilosus BCRC38072. The entire nucleotide sequence of the MRT element was 5.5-kb long, including two open reading frames. These two ORFs showed homologies to gag-like and pol-like gene products, and an A-rich sequence at the 3' end of pol-like gene. ORF1 encoded a protein of 517 amino acids and contained a cysteine-rich zinc finger motif. ORF2 encoded a protein of 1181 amino acids and contained apurinic/apyrimidinic endonuclease (APE), reverse transcriptase (RT), RNaseH domains, and a CCHC motif. The phylogenetic analyses demonstrated that the MRT element should be classified into the Tad1 clade. The results of Southern hybridizations showed that MRT elements were distributed within M. pilosus, M. ruber, M. sanguineus, and M. barkeri. In addition, the species of Monascus can be grouped by the presence or absence of MRT elements in the hybridization pattern according to phylogenetic subgroups established with the partial β-tubulin gene. |
本系統中英文摘要資訊取自各篇刊載內容。