查詢結果分析
相關文獻
- Arabidopsis HIT1, a Putative Homolog of Yeast Tethering Protein Vps53p, is Required for Pollen Tube Elongation
- Characterization of the Thylakoid Membrane in a Chlorophyll-Deficient ch5 Mutant of Arabidopsis Thaliana
- 苦瓜果實構造及發育之研究(Ⅰ)--苦瓜果實構造與受精過程
- 應用花粉管基因導入法輔助甘藍抗黑腐病育種之研究
- Interspecific Crosses of Lily by in Vitro Pollinated Ovules
- 茶樹自交不親和現象之觀察
- 植物真菌病原之初期侵染行為
- 應用花粉管法轉移甘藍基因注射處理時間之探討
- 水溶性擴散物對茶樹自交不親和性之影響
- Promoter Activity of a Soybean Gene Encoding a Seed Maturation Protein, GmPM9
頁籤選單縮合
題名 | Arabidopsis HIT1, a Putative Homolog of Yeast Tethering Protein Vps53p, is Required for Pollen Tube Elongation=囊泡繫鏈同源蛋白質HIT1為阿拉伯芥花粉管伸長之必要因子 |
---|---|
作者 | 王亮晴; 葉靖輝; 李雅韻; 陸重安; 吳少傑; | 書刊名 | Botanical Studies |
卷期 | 49:1 2008.01[民97.01] |
頁次 | 頁25-32 |
分類號 | 373.3 |
關鍵詞 | 阿拉伯芥; 花粉管; 尖端生長; 囊泡繫鏈因子; Arobidopsis thaliana; Pollen tube; Tip growth; Vesicle tethering factor; Yeast Vps53p protein; |
語文 | 英文(English) |
中文摘要 | 阿拉伯芥HIT1基因所編碼之蛋白質,與酵母菌囊泡繫鏈蛋白質Vps53p有同源性。由乙基甲基磺酸酯(Ethyl methane sulfonate)所造成之點突變對偶基因hit1-1,可以以同型合子(homozygous)的方式存在。帶有T-DNA插入之對偶基因 hit1-2及hit1-3,則只能以半合子(hemizygous)的方式存在。將野生種與突變種進行正反交實驗,結果顯示hit1-2及hit1-3 只能以半合子的方式存在,起因於雄配子體的缺陷。進一步以HIT1啟動子嵌合GUS報導基因做實驗,結果顯示在生殖器官的部份,HIT1基因的表現主要是在成熟的花粉粒及延伸的花粉管。以離體方式量測花粉管的發育,顯示來自於hit1-1/hit1-1同型合子植株之花粉粒,可以長出花粉管,但長度較野生種之花粉管為短。來自於HIT1/hit1-2或HIT1/hit1-3異型合子植株之花粉粒,約半數可長出長度近似野生種之花粉管,其餘之花粉粒則無法發育出花粉管。由上述之結果推知,HIT1可能參與了某花粉管尖端生長(tip growth)所必須之囊泡運送,該運送機轉若因HIT1的損傷而失去作用,將造成雄配子體之不孕。此外,hit1-1應為部分功能喪失之對偶基因,而 hit1-2及hit1-3則為無工能之對偶基因。 |
英文摘要 | The Arabidopsis HIT1 gene encodes a protein that is homologous to the yeast tethering factor Vps53p, which is involved in retrograde vesicle trafficking from the endosome to the trans-Golgi network. Although the ethyl methanesulfonate mutagenized hit1-1 allele can be maintained homozygously, T-DNA insertional hit1-2 and hit1-3 mutants can only be isolated as hemizygous lines. No heterozygous progeny were produced in outcrosses to wild-type plants using pollen from either hit1-2 or hit1-3 heterozygotes. The reciprocal cross using pollen from wild-type plants on either hit1-2 or hit1-3 mutants produced heterozygous and wild-type progeny. In reproductive tissues, HIT1 promoter-driven GUS activity was detected only in mature pollen and elongated pollen tubes. In vitro pollen germination further showed that only half the pollen grains from hit1-2 and hit1-3 heterozygote plants produced normal pollen tubes. In contrast, the pollen tube length of pollen grains from the hit1-1 mutant was reduced compared to that of the wild type. These results suggest that HIT1 may govern a vesicle trafficking event that is required for pollen tube tip growth during male gametogenesis and that disruption of HIT1 results in male specific transmission defect. Moreover, while the hit1-1 mutant is partially functional leading to reduced pollen tube length, hit1-2 and hit1-3 are total-loss-of-function alleles. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。