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題名 | Interspecific Crosses of Lily by in Vitro Pollinated Ovules=藉胚珠體外授粉法進行百合種間之雜交 |
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作者 | 紀海珊; | 書刊名 | Botanical Bulletin of Academia Sinica |
卷期 | 41:2 2000.04[民89.04] |
頁次 | 頁143-149 |
分類號 | 435.456 |
關鍵詞 | 種間雜交; 體外胚珠授粉; 百合屬; 穿入胚珠之珠孔; 花粉管; 助細胞; 精細胞; In vitro ovular pollination; Interspecific crosses; Lilium; Micropylar penetration; Pollen tube; Sperm cells; Synergid; |
語文 | 英文(English) |
中文摘要 | 由於受精前後之障礙,以亞洲型百合為母本,東方型百合或鐵炮百合為父本之組合的種間雜交後裔從未有成功的例子被報導過。本研究用體外胚珠受粉法使花粉管直接進入體外培養之胚珠內。獲得下列幾點結果:(1) 在體外培養中,胚珠與花粉間最適當之授粉距離為 1 公釐;(2) 種間雜交之障礙並不存在於花粉管穿入胚珠之珠孔處;(3) 以百合不同栽培種進行種內和種間之正反雜交,其花粉管都能正常的穿入胚珠之珠孔;(4) 在體外胚珠受粉法中發生多個花粉管進入同一胚珠珠孔之現象,而這些花粉管只經過胚囊外圍,並沒有進入胚囊之助細胞;(5) 以亞洲型百合為母本與東方型百合之雜交組合中,有少數花粉管進入胚囊之助細胞,並在進入胚囊之助細胞後釋放出花粉管之內含物;( 6) 花粉管穿入胚珠之珠孔所需的時間 (花粉發芽後 8-12 小時) 早於花粉管內精子形成的時間 (花粉發芽後 17 小時),此或許為體外胚珠授粉法受精失敗的可能原因。 |
英文摘要 | Because of pre- and post-fertilization barriers, successful interspecific crosses have not been reported for Lilium using Asiatic hybrid as the mother to cross with Oriental or Lilium longiflorum. Using an in vitro ovular pollination method, where pollen tubes directly penetrate isolated ovules, this study demonstrated that 1) the Brewbaker and Kwack agar plate containing sucrose (10%), with the isolated ovules being placed ca. 1 mm apart from the pollen, is optimal for pollination, 2) interspecific crossing barriers do not occur during penetration of the pollen tubes into the micropyle, 3) micropylar penetration can be obtained in all kinds of intra- and inter-specific cross combinations of lily cultivars, 4) multiple penetration occurs, in which the penetrating pollen tubes pass the embryo sac without entering the synergid cells, 5) a small amount of pollen tube penetrates the synergid cells in the A x O cross, and 6) completion of pollen tube penetration (8-12 h after germination) is much earlier than the division of the generative cell in sperm cells (17 h after germination), which may be the primary cause for the failure in fertilization. |
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