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題 名 | 利用cDNA微陣列來建構一個分子生物層級的中草藥分析平臺總報告=Constructing a Chinese Medicine Molecular Analysis Plateform by Utilizing cDNA Microarray |
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作 者 | 許志楧; | 書刊名 | 中醫藥年報 |
卷 期 | 25:2 2007.10[民96.10] |
頁 次 | 頁373-409 |
專 輯 | 中醫藥基因體及免疫學研究 |
分類號 | 414.33 |
關鍵詞 | 冬蟲夏草; cDNA微陣列; 分子基因圖譜; 化學指紋圖譜; 標記基因; Cordyeps sinensis; cDNA microarray; Molecular genetic fingerprinting; Chemical fingerprinting; Marker genes; Standardization; |
語 文 | 中文(Chinese) |
中文摘要 | 本三年計畫的目標為建立一個整合性的指紋圖譜平臺(Integrated fingerprinting platform)來作為中草藥標準化的依據。首先,我們使用分子基因圖譜分析(Molecular genetic fingerprint)來確定中草藥樣本冬蟲夏草的菌種。接著,利用一連串的化學分析來建立冬蟲夏草樣本的有機及無機化學指紋圖譜(Chemical fingerprint),以檢測冬蟲夏草樣本間化學成分的相似程度以及評估食用冬蟲夏草安全性。最後,我們進行了迴圈設計(Loop design)的微陣列實驗,並以統計的方法來分析冬蟲夏草的微陣列指紋圖譜(Microarray fingerprint)。此微陣列實驗的結果並進一步的以統計方法以及同步定量聚合反應(Real-time quantitative PCR)來驗證。我們也篩選出冬蟲夏草的標記基因並探討其可能的藥理機制。在此,我們證實了:一個經過嚴謹設計並驗證過的cDNA微陣列技術平臺,可以有效的區分出化學成分十分類似的中草藥樣本。這個整合性的指紋圖譜平臺可以作為研究中草藥的標準化的流程,並且可作為將來中醫藥臨床研究的參考。 |
英文摘要 | In this three-year study, we aim to develop a standardization protocol for the study of Chinese herbal formulas by integrating three fingerprinting platforms. First, we performed molecular genetic fingerprinting study to assure the authenticity of C. sinensis samples. Then, a series of organic and inorganic chemical fingerprinting analysis were carried out to monitor the similarity of chemical profiles between samples and to evaluate the safety of C. sinensis raw material. Finally, a set of loop-design microarray experiments was conducted and microarray fingerprints of various Cordyceps samples were analyzed by statistical calculation. The quality of microarray data was confirmed by both statistical methods and real-time PCR experiment. Specific C. sinensis marker genes are identified and the underlying pharmacological mechanisms are discussed. Here, we further demonstrated that a carefully designed and validated microarray fingerprinting platform is able to molecularly differentiate distinct C. sinensis samples with minute chemical differences. This integrated fingerprinting platform could be implemented to the standardization as well as the future in vivo study on traditional Chinese herbal formulas. |
本系統中英文摘要資訊取自各篇刊載內容。